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为全面了解Ｎ－ｒａｓ癌基因突变与急性髓细胞白血病（ＡＭＬ）亚型的关系，从新鲜骨髓和库存骨髓片提取ＤＮＡ，应用银染聚合酶链反应－单链构型多态性（ＰＣＲ－ＳＳＣＰ）技术对９８例ＡＭＬ患者的Ｎ－ｒａｓ癌基因第１和第２外显子的点突变进行了研究。结果显示：共有２９例检测到突变，其中第１和第２外显子突变分别为１８例和１４例，两外显子同时突变３例；突变率为２９．５９％。除Ｍ［７］未检测外，其它各亚型均发现突变；而以Ｍ４ａ为多见，Ｍ［３ａ］为少见。因此，Ｎ－ｒａｓ癌基因突变是ＡＭＬ常见的肿瘤标志。
In order to fully understand the relationship between N-ras oncogene mutation and acute myeloid leukemia (AML) subtypes, DNA was extracted from fresh bone marrow and stock bone marrow using silver-staining and single-strand conformation polymorphism (PCR- SSCP) technique was used to investigate point mutations in exon 1 and exon 2 of N-ras oncogene in 98 AML patients. The results showed that: A total of 29 cases detected mutations, of which 1 and 2 exons mutations were 18 cases and 14 cases, two exons simultaneously mutation in 3 cases; mutation rate was 29.59%. Mutations were found in all other subtypes except M [7], whereas M4a was more common and M [3a] was rare. Therefore, N-ras oncogene mutation is a common tumor marker of AML.