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目的探索副溶血性弧菌快速检测法,应用于日常监测及食物中毒的快速查源。方法用副溶血性弧菌实时荧光试剂盒对水产品样本进行检验,以副溶血性弧菌toxR基因为靶序列,设计1对引物和探针,采用热裂解法提取DNA。结果实时荧光PCR从42份水产品样品的增菌液中检出13份样品副溶血性弧菌阳性,与传统培养法相比一致性极好(K=0.943,K>0.75)。结论实时荧光PCR方法在副溶血性弧菌的检验方面较传统方法具有快速、灵敏、特异性强等优势,具有广阔的应用前景。
Objective To explore the rapid detection of Vibrio parahaemolyticus, which is used in routine monitoring and rapid investigation of food poisoning. Methods The samples of aquatic products were tested with the Vibrio parahaemolyticus Real-time Fluorescent Kit, and a pair of primers and probes were designed using toxR gene of Vibrio parahaemolyticus as target sequence. DNA was extracted by thermal pyrolysis. Results Real-time PCR showed that 13 samples were positive for Vibrio parahaemolyticus from the enrichment broth of 42 samples of aquatic products, which showed excellent consistency (K = 0.943, K> 0.75) compared with the traditional culture method. Conclusion Real-time fluorescence PCR has the advantages of rapid, sensitive and specific in the detection of Vibrio parahaemolyticus, which has a broad application prospect.