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目的鉴定吉林省结核分枝杆菌(MTB)中的北京基因型及其与耐药性的关联。方法 95株结核分枝杆菌分离自2008-2009年吉林省临床肺结核病人的痰液标本,培养方法为改良酸性罗氏(L-J)培养基。分离出的抗酸杆菌经对硝基苯甲酸(PNB)培养基初步鉴定后选出结核分枝杆菌复合群并应用比例法进行药敏试验(DST)。北京基因型的鉴定采用RD105基因缺失法。实验结果应用Epi Info统计软件和卡方检验进行分析。结果 95株结核分枝杆菌中,北京基因型占88.4%(84/95)。北京基因型中,异烟肼、利福平、乙胺丁醇、链霉素、卡那霉素和氧氟沙星的耐药率分别为31.7%、23.2%、15.9%、31.7%、13.4%和18.3%,均位于非北京基因型菌株耐药率的95%置信区间内。北京基因型中的多重耐药(MDR)结核(TB)率占19.5%,通过卡方检验与非北京基因型中的MDRMTB进行比较,差异无统计学意义(P>0.05)。结论 RD105基因缺失法是鉴定MTB北京基因型的简便、有效方法;北京基因型为吉林省结核分枝杆菌的优势株;北京基因型与非北京基因型耐药率无统计学意义上的差别。
Objective To identify Beijing genotypes of Mycobacterium tuberculosis (MTB) in Jilin Province and their association with drug resistance. Methods Ninety-five Mycobacterium tuberculosis isolates were isolated from sputum samples of clinical pulmonary tuberculosis patients in Jilin Province during 2008-2009. The culture method was modified L-J medium. The isolated acid-fast bacilli were initially identified by p-nitrobenzoic acid (PNB) medium, and the Mycobacterium tuberculosis complex was selected and the drug susceptibility test (DST) was performed using the proportional method. Beijing genotype identification using RD105 gene deletion method. The results of the experiment using Epi Info statistical software and chi-square test for analysis. Results Among the 95 strains of Mycobacterium tuberculosis, Beijing genotype accounted for 88.4% (84/95). In Beijing genotype, the resistance rates of isoniazid, rifampin, ethambutol, streptomycin, kanamycin and ofloxacin were 31.7%, 23.2%, 15.9%, 31.7%, 13.4 % And 18.3%, respectively, were all within 95% confidence intervals of the non-Beijing genotypes. The MDR TB rate in Beijing genotype accounted for 19.5%. There was no significant difference (P> 0.05) between the MDRMTB and non-Beijing genotype by chi-square test. Conclusion RD105 gene deletion method is a simple and effective method to identify MTB Beijing genotype. Beijing genotype is the dominant strain of Mycobacterium tuberculosis in Jilin province. There is no statistically significant difference between Beijing genotypes and non-Beijing genotypes.