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目的 :探讨银杏叶提取物 (ExtractofGinkgobiloba,EGb)对豚鼠游离结肠平滑肌细胞的直接作用机制。方法 :用含 1g/LⅠ型胶原酶及 0 .1g/L大豆胰蛋白酶抑制剂的消化液 ,消化获得游离的豚鼠结肠平滑肌细胞 ,将不同浓度EGb加入细胞悬液中 ,用显微镜测微尺随机观察 50个细胞长轴变化情况 ,计算用药前后细胞平均长度。如果 :EGb对结肠平滑肌细胞具有舒张作用 ,在 0 .1 1～ 3 .5g/L浓度范围内 ,EGb对结肠平滑肌细胞的作用呈剂量 -效应关系 ,最大反映浓度为 1 .75g/L ,其舒张效应为 1 2 .79% (P <0 .0 5)。EGb预先孵育 1min ,可抑制乙酰胆碱 (ACh)效应的 44 .1 3 % (P <0 .0 1 )。腺苷酸环化酶激动剂Forskolin可增强EGb的舒张效应 ,与单独应用EGb组相比较 ,舒张百分数增加 8.87% (P <0 .0 5)。结论 :EGb对豚鼠游离结肠平滑肌细胞具有直接作用 ,这种作用可能与cAMP信号传导通路相关
Objective: To investigate the direct mechanism of Ginkgo biloba extract (EGb) on guinea pig free colonic smooth muscle cells. Methods: Divide the guinea pig colonic smooth muscle cells with digestive fluid containing 1g/L type collagenase and 0.1g/L soybean trypsin inhibitor, and add different concentrations of EGb to the cell suspension. Observe changes in the long axis of 50 cells and calculate the average cell length before and after administration. If: EGb has a relaxing effect on colonic smooth muscle cells, in the concentration range of 0.11 to 3.5g/L, the effect of EGb on colonic smooth muscle cells is dose-effect relationship, the maximum reflected concentration is 1.75g/L. The diastolic effect was 12.79% (P < 0.05). Preincubation with EGb for 1 min inhibited the ACh effect by 44.13% (P < 0.01). Forskolin, an adenylate cyclase agonist, increased the EGb relaxation, and the diastolic percentage increased by 8.87% (P < 0.05) compared with the EGb alone group. Conclusion: EGb has a direct effect on guinea pig free colonic smooth muscle cells, which may be related to cAMP signaling pathway