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目的 探讨多囊卵巢综合征 (PCOS)患者血管紧张素转换酶 (ACE)基因多态性与肾素血管紧张素系统 (RAS)的关系 ,了解RAS在PCOS发病中的作用。方法 采用聚合酶链反应 (PCR)方法 ,检测PCOS患者和对照组ACE基因插入和 (或 )缺失多态性 ;采用放射免疫方法测定两组血清醛固酮 (ALD)水平 ,比较缺失型等位基因与ALD的关系。结果 PCOS患者ALD水平显著高于对照组 ,PCOS患者D等位基因组ALD水平显著高于I等位基因组。结论 PCOS患者D等位基因的存在与RAS功能亢进有关 ,RAS的产物血管紧张素Ⅱ (ATⅡ )可使卵泡在各阶段闭锁 ,导致不排卵 ,参与了多囊卵巢和高雄激素血症的形成。
Objective To investigate the relationship between angiotensin converting enzyme (ACE) gene polymorphism and renin angiotensin system (RAS) in patients with polycystic ovary syndrome (PCOS) and to investigate the role of RAS in the pathogenesis of PCOS. Methods Polymerase chain reaction (PCR) method was used to detect the insertion and / or deletion polymorphism of ACE gene in PCOS patients and controls. Serum aldosterone (ALD) levels were measured by radioimmunoassay, and the deletional alleles ALD relationship. Results The ALD level in PCOS patients was significantly higher than that in control subjects. The ALD level in D allele of PCOS patients was significantly higher than that in I alleles. Conclusions The presence of D allele in patients with PCOS is associated with hyperactivity of RAS. Angiotensin Ⅱ (AT Ⅱ), a product of RAS, can block the follicles at various stages and lead to anovulation and participate in the formation of polycystic ovary and hyperandrogenism.