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目的:研究附子生物碱对大肠杆菌和金黄色葡萄球菌生长代谢作用的影响,并对其抗菌活性进行评价。创新点:首次应用微量热的方法研究了大肠杆菌和金黄色葡萄球菌在附子生物碱中的生长代谢作用,并从热力学角度对附子生物碱的抗菌活性进行了评价。方法:微量量热仪绘制了37°C时大肠杆菌和金黄色葡萄球菌在不同浓度的附子生物碱作用下的热谱曲线,根据热动力学模型,分别得到了生长速率常数μ、抑制率I、最大产热功率Pm以及最大产热功率所对应的时间Tm等热动力学参数,从热动力学的角度探讨了附子生物碱对大肠杆菌和金黄色葡萄球菌代谢作用的影响。结论:实验结果显示,附子生物碱对大肠杆菌的生长代谢基本没有影响。但是对于金黄色葡萄球菌,随着附子生物碱浓度的增大,其生长速率常数呈现先升高后降低的趋势,同时达峰时间也被延长,说明附子生物碱对金黄色葡萄球菌存在潜在的生长抑制作用。
Objective: To study the effect of aconite alkaloids on the growth and metabolism of Escherichia coli and Staphylococcus aureus, and to evaluate its antibacterial activity. Innovative point: the first application of calorimetry Escherichia coli and Staphylococcus aureus in the growth and metabolism of aconite alkaloids, and from the thermodynamic point of view of the antibacterial activity of aconite alkaloids were evaluated. Methods: The thermogram of Escherichia coli and Staphylococcus aureus at 37 ° C under different concentrations of aconite alkaloids was plotted. According to the thermokinetic model, the growth rate constant μ, the inhibition rate I , The maximum heat production power (Pm) and the time Tm corresponding to the maximum heat production power, the effects of aconite alkaloids on the metabolism of Escherichia coli and Staphylococcus aureus were discussed from the perspective of thermodynamics. Conclusion: The experimental results show that the aconite alkaloids on the growth and metabolism of E. coli has basically no effect. But for Staphylococcus aureus, with the concentration of aconite alkaloids increased, the growth rate constant first increased and then decreased, and the peak time was also extended, indicating the existence of aconite alkaloids Staphylococcus aureus potential Growth inhibition.