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目的:探讨四逆散对实验性溃疡性结肠炎(UC)大鼠白介素(IL)-6和IL-13的影响,以及君臣配伍在组方中的作用。方法:SD大鼠50只,分为5组,即正常组、模型组、四逆散组、柴芍枳组和柴芍组;采用免疫法造模,用家兔新鲜结肠黏膜制备抗原乳化液,分别于造模第1,10,17,24天注射于大鼠的双侧足跖、腹股沟及背部,造模第2天分组ig,正常组与模型组按照10 mL·kg-1蒸馏水ig;四逆散组按照1.75 g·kg-1四逆散溶液ig;柴芍枳组按照1.31 g·kg-1ig;柴芍组按照0.88 g·kg-1ig;第29天ig后,取材,处死大鼠;采用双抗体夹心ELISA法检测大鼠血清IL-6和IL-13水平。结果:模型组大鼠血清IL-6(163.64±22.17)μg·L-1明显高于正常组(59.80±11.72)μg·L-1,(P<0.05),IL-13(78.64±12.66)μg·L-1显著低于正常组(616.00±133.80)μg·L-1,(P<0.05);与模型组比较,四逆散能显著降低大鼠血清IL-6含量(73.00±9.92)μg·L-1,明显增加IL-13含量(264.89±128.05)μg·L-1(P<0.05);去掉甘草的柴芍枳配伍只降低大鼠血清IL-6含量(131.75±31.93)μg·L-1(P<0.05),对IL-13含量无明显影响;而柴芍配伍对IL-6,IL-13含量无明显影响。结论:四逆散干预实验性UC的机制与调控细胞因子网络平衡有关,对实验性UC大鼠血清IL-6,IL-13的影响以四逆散全方作用最佳,而方中柴芍的君臣配伍并未发挥主要作用。
Objective: To investigate the effects of Sini Decoction on interleukin (IL) -6 and IL-13 in rats with experimental ulcerative colitis (UC) and the effect of monarch and sage on the rats. Methods: Fifty SD rats were randomly divided into 5 groups: normal group, model group, Sini Powder group, Chaishaozhi group and Chaishao group. Immunohistochemical method was used to prepare the antigen colloids of rabbits with fresh colonic mucosa Were injected into the bilateral paws, the groin and the back of the rats on the 1st, 10th, 17th and 24th day respectively. The rats were divided into two groups on the second day after modeling. The normal group and the model group were treated with 10 mL · kg -1 distilled water ig ; Sini powder group according to 1.75 g · kg-1 Sini solution ig; Chaihao orange group according to 1.31 g · kg-1ig; Chai paeons group according to 0.88 g · kg-1ig; 29 days after ig, drawing, sacrifice Rats; serum IL-6 and IL-13 levels were detected by double antibody sandwich ELISA. Results: The level of IL-6 in the model group was significantly higher than that in the normal group (163.64 ± 22.17 μg · L-1, 59.80 ± 11.72 μg · L-1, P <0.05, IL-13, 78.64 ± 12.66, μg · L-1 was significantly lower than that of the normal group (616.00 ± 133.80) μg · L-1, P <0.05). Compared with the model group, Sini Decoction significantly decreased the level of IL-6 in serum (73.00 ± 9.92) (P <0.05). IL-6 content in serum was significantly lower than that in control group (131.75 ± 31.93) μg / L · L-1 and 264.89 ± 128.05 μg · L- · L-1 (P <0.05), but had no significant effect on the content of IL-13. However, the compatibility of Chai Pao was not affected by IL-6 and IL-13. CONCLUSIONS: The mechanism of Sini Powder intervention on experimental UC is related to the regulation of cytokine network balance. The effect of Sini Powder on all-round effect is best on serum IL-6 and IL-13 in experimental UC rats. The combination of monarch and minister did not play a major role.