目的 :野葛毛状根的诱导和无激素离体培养。方法 :用含超强毒性基因的发根农杆菌R16 0 1与野葛离体叶片共培养。结果 :经感染的野葛叶片表面直接形成大量生长快、分枝多、负向地性的毛状根 ,毛状根离体培养具有抗卡那霉素特性和激素自给特性。结论 :建立了发根农杆菌诱导野葛毛状根的方法和野葛毛状根的离体培养系统。 Objective : The induction of hairy roots of Pueraria lobata and hormone-free culture in vitro. Methods: Co-cultivation of Agrobacterium rhizogenes R16 01 containing super-virulent genes and isolated leaves of Kudzu. RESULTS: A large number of hairy roots with fast growth, multiple branching, and negative geochemistry were directly formed on the surface of infected leaves of Pueraria lobata. Hairy root culture had the characteristics of anti-kanamycin resistance and hormone self-feeding. Conclusion: The method of inducing A. rhizogenes hairy roots and the in vitro culture system of hairy roots of Pueraria lobata were established.