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文章用不同浓度的乙醇作为浸提剂,对药用植物新疆天仙子茎段部分的总黄酮成分进行分离提取,并运用分光光度法对总黄酮进行了含量测定。实验结果表明,温度在40℃,量液比为1:10之下,用70%乙醇浸泡2小时左右能对总黄酮有较理想的提取(1.32%)。滤纸片扩散法进行的抑菌试验结果显示,新疆天仙子的总黄酮提取物对大肠杆菌具有一定的抑菌活性(抑菌圈直径12.5mm),而对枯草杆菌、金黄色葡萄球菌和短小杆菌没有表现出明显的抑菌作用。
In this paper, different concentrations of ethanol as extractants were used to separate and extract the total flavonoids from the stem segments of Medicago sativa L., and the content of total flavonoids was determined by spectrophotometry. The experimental results showed that the optimum extraction conditions of total flavonoids (1.32%) were obtained by soaking in 70% ethanol for 2 hours at the temperature of 40 ℃ and the liquid to liquid ratio of 1:10. Bacteriostatic test carried out by the filter paper diffusion method showed that the total flavonoids extract of Angelica sinensis had a certain antibacterial activity against Escherichia coli (bacteriostatic circle diameter 12.5mm), while Bacillus subtilis, Staphylococcus aureus and Bacillus brevis Did not show obvious bacteriostasis.