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AIM:To investigate the immunogenicity of a novel DNA vaccine,pSW3891/HBc, based on HBV core gene in Balb/c mice. METHODS:A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. Balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay. RESULTS:HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97 200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW389l/HBc DNA vaccine. CONCLUSION:pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific CTL response in immunized Balb/c mice.
AIM: To investigate the immunogenicity of a novel DNA vaccine, pSW3891 / HBc, based on HBV core gene in Balb / c mice. METHODS: A novel DNA vaccine, pSW3891 / HBc, encoding HBV core gene constructed using a plasmid pSW3891. Balb / c mice were immunized with either pSW3891 / HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay. RESULTS: HBcAg was effectively expressed in 293T cell line transiently transfected with pSW3891 / HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891 / HBc. The end-point titers of anti-HBc reached the highest 1:97 200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector: target ratio of 20: 1 in mice that received pSW389l / HBc DNA vaccine. CONCLUSION: pSW3891 / HBc vaccination elicits specific anti- HBc r esponse and induces HBc-specific CTL response in immunized Balb / c mice.