【摘 要】
：
Meiotic recombination is essential for reciprocal exchange of genetic information between homologous chromosomes and their subsequent proper segregation in sexually reproducing organisms.MLH1 and MLH3 belong to meiosis-specific members of the MutL-homoiog
【机 构】
：
National Key Laboratory of Crop Genetic Improvement,National Center of Plant Gene Research (Wuhan),H
论文部分内容阅读
Meiotic recombination is essential for reciprocal exchange of genetic information between homologous chromosomes and their subsequent proper segregation in sexually reproducing organisms.MLH1 and MLH3 belong to meiosis-specific members of the MutL-homoiog family,which are required for normal level of crossovers (COs) in some eukaryotes.However,their functions in plants need to be further elucidated.Here,we report the identification of OsMLH1 and reveal its functions during meiosis in rice.Using CRISPR-Cas9 approach,two independent mutants,Osmlh1-1 and Osmlh1-2,are generated and exhibited signifi-cantly reduced male fertility.In Osmlh1-1,the clearance of PAIR2 is delayed and partial ZEP1 proteins are not loaded into the chromosomes,which might be due to the deficient in resolution of interlocks at late zygotene.Thus,OsMLH1 is required for the assembly of synapsis complex.In Osmlh1-1,CO number is dropped by ～53％ and the distribution of residual COs is consistent with predicted Poisson distribution,indicating that OsMLH1 is essential for the formation of interference-sensitive COs (class I COs).OsMLH1 interacts with OsMLH3 through their C-terminal domains.Mutation in OsMLH3 also affects the pollen fertility.Thus,our experiments reveal that the conserved heterodimer MutLγ (OsMLH1-OsMLH3) is essential for the formation of class I COs in rice.
其他文献
针对半潜式起重拆解平台的排载作业需求,提出水泵与空压机相结合的联合排载方式。建立排载系统的数学模型,在SimuWorks仿真环境中搭建仿真模型,对双机起吊0~4200 t典型起重工况进行仿真试验。分析联合排载方式下立柱压载舱内空气压力、液位和海水流量的变化情况,比较空压机快速排载、水泵排载、联合排载等3种方式的优劣。仿真试验结果表明,联合排载法能更好地兼顾平台稳性、安全性与快速性。
基于有限元方法对阵列排布的隔水导管组成的群桩基础开展竖向载荷作用下的破坏模式研究,分析不同井口间距、桩数、桩径和桩长下隔水导管复合群桩基础的极限承载力和轴向载荷传递规律,结合隔水导管的结构特点,推导适用于隔水导管复合桩的群桩效应系数计算公式。研究表明:隔水导管复合桩群桩效应系数随距径比Sa/D1的增大、桩数N的减小、桩径D1的增大及不排水强度Su的增大而增大;增加变截面以下桩长L2,可提高复合群桩
采用故障模式和影响分析(Failure Mode and Effect Analysis,FMEA)方法,以快速压排载系统为研究对象,对其涉及的压载系统、液位遥测系统、舱底水系统、集成控制与监测系统(Integrated Control and Monitoring System,IAS)和阀门遥控系统进行深入分析。结果表明,舱底水系统的潜在风险较大,为整个快速压排载系统最薄弱的环节,液位遥测系统则为系统中可靠性较高的子系统。
一、题目的来源 2007年是广东新课改的新的一轮高考的第一年，综观2007年高考化学广东卷，亮点频频显现，处处体现新课改、新理论，以学生为本，以教学为源，彰显素质教育之根本，可谓是“此时无声胜有声”，令人赏心悦目、回味无穷。其中第27题最值本人欣赏。 二、原题及解答 原题是：克矽平是一种治疗矽肺病的药物，其合成路线如下（反应均在一定条件下进行） （1）化合物I的某些性质类似苯。例如，化合物
针对影响夹具密封能力的相关因素开展分析。影响夹具周向密封能力的因素包括密封圈与管道间距离、摩擦因数、分割环与管道间距离。影响夹具轴向密封能力的因素为摩擦因数。基于夹具密封结构,利用有限元软件Abaqus对影响密封能力的因素开展有限元建模及模拟计算,并对模拟计算结果开展分析。摩擦因数、分隔圈与管道间距离、密封圈与管道间距离的增大不利于增强夹具的周向密封能力,设计时可适当减小相关参数。摩擦因数对夹具的轴向密封能力基本没有影响,设计时可忽略摩擦因数对轴向密封的影响。所开展的工作能够为后续海底油气管道夹具密封结构
针对螺旋桨激励引起的海洋平台上层建筑低频线谱振动问题,基于动力吸振理论,开展海洋平台分布式动力吸振研究。建立四自由度主从耦合动力吸振模型,分析动力吸振器吸振性能随布放位置的变化规律;研究分布式动力吸振器数量和布放方案对板架结构减振效果的影响;以某海洋平台为例,开展分布式动力吸振的应用研究,降低海洋平台上层建筑区域的振动响应。研究表明:当动力吸振器总质量不变时,吸振器布放位置对减振效果影响较大;当分布式动力吸振器质量分配合理时,其减振效果优于单点动力吸振器。
Wild progenitors are an excellent source for strengthening the genetic basis and accumulation of desirable variation lost because of directional selection and adaptation in modern cultivars.Here,we re-evaluate a landrace of Gossypium hirsutum,formerly kno
Airway smooth muscle (ASM) has developed a mechanical adaption mechanism by which it transduces force and responds to environmental forces,which is essential for periodic breathing.Cytoskeletal reor-ganization has been implicated in this process,but the r
针对35万t浮式生产储卸油装置(Floating Production Storage and Offloading,FPSO)模块特点,对超长超窄超高管廊模块、电气模块、船体与模块连接节点、信息化等方面进行研究,提炼分段建造、一体化建造、运输框架设计、连接节点工艺、精细化管理流程等建造关键技术,并阐述模块建造的其他事项,为后续类似项目提供技术参考。
On February 3rd,2020,an isolated Ms5.1 earthquake occurred in the northern section of the Longquanshan fault zone.This study aims at defining the geometry of seismogenic structures of this earthquake.In detail,centroid moment tensor inversion results show