论文部分内容阅读
目的 研究用受体血浆冷冻处理的异体神经移植 ,以提高神经再生效果。方法 选用 16条家狗的双侧正中神经 ( 3 2条 ) ,作成双侧正中神经缺损 3cm的模型。 ( 1)实验组 :左前肢用受体血浆冷冻处理的同种异体神经缝接于神经缺损处。 ( 2 )对照组 :右肢缺损处用冷冻处理的同种异体神经桥接。两组于术后 7、2 1、90、180d不同时间点 ,分别进行组织学观察和图像分析仪测定。结果 术后随时间的延长 ,实验组神经纤维再生数量明显较对照组多 ,而神经纤维间的结缔组织胶原原纤维远较对照组要少。结论 神经缺损用经受体血浆冷冻处理的同种异体神经移植后 ,神经再生效果明显优于单纯冷冻异体神经移植。
OBJECTIVE: To study the effects of nerve allograft with receptor plasma freezing in order to improve the effect of nerve regeneration. Methods Bilateral median nerves (32) of 16 domestic dogs were selected to make a 3 cm bilateral median nerve defect. (1) Experimental group: Allogeneic nerves frozen in the left forelimb with recipient plasma were stitched to the nerve defect. (2) Control group: Allogeneic nerve bridging with cryopreservation at right limb defect. At 2, 7, 1, 90 and 180 days after operation, histological observation and image analyzer were performed respectively. Results With the extension of time after surgery, the number of nerve fiber regeneration in the experimental group was significantly more than that in the control group, while the collagen fibers in the nerve fibers were much less than those in the control group. Conclusions After nerve allograft with neurological deficit treated by plasma cryotherapy, the effect of nerve regeneration is better than that of pure cryo-allograft.