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本文报道了用薄层扫描仪对杂交水稻F_1种子进行荧光扫描,根据扫描波形来检出F_1种子,检出的F_1种子再用酯酶同工酶电泳法验证。对汕优2号的检出率为62~68%,对威优6号检出率为63~66%,对汕优6号的检出率为48~52%,汕优61选检出率为49~52%,对人为掺杂威优64的检出率达100%,误检情况只偶尔出现。
In this paper, F_1 seeds of hybrid rice were scanned by TLC scanner and F_1 seeds were detected by scanning waveforms. The detected F_1 seeds were verified by esterase isoenzyme electrophoresis. The detection rate of Shanyou 2 was 62-68%, that of Weiyou 6 was 63-66%, that of Shanyou 6 was 48-52%, and that of Shanyou 61 The rate is 49 ~ 52%, the detection rate of artificial doping Wei You 64 is 100%, and the false positives happen only occasionally.