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为了从转录和翻译两个水平上增加Ｂ．ｔ．ＣｒｙＩＡ基因在转基因植物中的表达，一方面利用ＰＣｇｅｎｅ对该基因进行了全面重新设计，删除了前面一小段和后面一大段对杀虫效果没有影响的氨基酸顺序，对剩余的约２ｋｂ的基因不改变氨基酸序列，只改变ＤＮＡ序列，改造原则主要为两点：消除引起ｍＲＮＡ不稳定的因素和采用适宜于植物中表达的ｃｏｄｏｎｕｓａｇｅ，通过对每一个氨基酸密码子进行分析，直到确定整个基因序列；另一方面我们把改造后的Ｂ．ｔ．基因构建在两种适宜于不同植物的强化表达载体上，目前已完成设计、改造及建构工作。
To increase B from both transcriptional and translational levels. t. CryIA gene expression in transgenic plants, on the one hand the PCgene gene was completely redesigned to delete the first and last large section of the amino acid sequence has no effect on the insecticidal effect of the remaining about 2kb of the gene is not Change the amino acid sequence, only change the DNA sequence, the principle of transformation is mainly two points: to eliminate the factors that cause mRNA instability and codonusage suitable for expression in plants by analyzing each amino acid codon, until the entire gene sequence is determined; On the one hand we have transformed B. t. Genes are built on two intensified expression vectors suitable for different plants and have been designed, engineered and constructed.