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应用高效液相色谱法测定大黄1—5年不同生长年限、植株不同发育阶段、植株不同部分中番泻式A(sennoside A,SA),大黄酸-8-葡萄糖甙(rhein-8-O-β-D-glucoside,R8G),大黄酸甙(rheinoside)A(RA),C(RC),D(RD)等五种蒽甙类成分的含量,结果表明:(1)大黄随生长年限的延长,五种蒽甙类成分的总含量呈递增趋势,但第二年以后增加缓慢;(2)不同发育阶段中五种蒽甙的总量以果熟期为最高;(3)大黄的根系中五种蒽甙的总含量依下列顺序递减:主根茎→细根→主根及支根→支根茎;茎部只含少量大黄酸甙 D,叶不含任何蒽甙类成分。本研究有利于确定大黄合理的采收年限和季节,以及大黄资源的合理利用。
Determination of sennoside A (SA) and rhein-8-O- in different growth stages of plants and their different developmental stages in different parts of rhubarb using high performance liquid chromatography β-D-glucoside, R8G), rheinoside A (RA), C (RC), D (RD) and other five anthraquinone components, the results show that: (1) rhubarb with growing years The total content of the five anthraquinones showed an increasing trend, but it increased slowly after the second year; (2) The total amount of the five earthworms in different developmental stages was the highest in the fruit ripening period; (3) The root system of rhubarb. The total content of the five species of midges decreased in the following order: main rhizome→thin root→major root and branch root→branched stem; the stem contained only a small amount of rheinate D, and the leaves did not contain any anthraquinone components. This study is conducive to determining the reasonable harvest age and season of rhubarb, and rational use of rhubarb resources.