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目的紫杉醇是一种高效的抗癌新药,对各种癌症具有显著疗效。紫杉醇药源不足问题是限制其广泛应用的关键。在众多紫杉醇的生产工艺中,微生物发酵法具有明显的优势。然而,目前微生物发酵法生产紫杉醇的产量还不能达到工业化生产的要求。因此,提高微生物发酵法生产紫杉醇的产量是亟待解决的问题。方法本试验中作者采用单因子试验研究了不同终浓度的苯甲酸钠对紫杉醇产生菌J1-3紫杉醇产量的影响。结果向S-7液体发酵培养基中添加终浓度为25 mg.L-1的苯甲酸钠能显著促进紫杉醇产生菌合成紫杉醇。结论苯甲酸钠是可以增加紫杉醇产量的有效前体,但如果剂量过大会阻碍紫杉醇的积累。
PURPOSE Paclitaxel is a potent anti-cancer drug with a significant effect on various cancers. The problem of lack of source of paclitaxel is the key to limit its wide application. In many paclitaxel production processes, microbial fermentation has obvious advantages. However, the current production of paclitaxel by microbial fermentation can not meet the requirements of industrialized production. Therefore, raising the production of paclitaxel by microbial fermentation is an urgent problem to be solved. Methods In this study, the authors used single factor experiments to study the effects of sodium benzoate at different final concentrations on the paclitaxel-producing strain paclitaxel production of paclitaxel J1-3. Results The addition of sodium benzoate to S-7 liquid fermentation medium at a final concentration of 25 mg.L-1 significantly promoted paclitaxel-producing bacteria to synthesize paclitaxel. Conclusions Sodium benzoate is a potent precursor that increases paclitaxel production, but obstructs paclitaxel accumulation if overdose.