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目的:研究在复苏前静脉输注ω-3多不饱和脂肪酸对失血性休克复苏后大鼠全身炎症反应的抑制以及对肺的保护作用。方法:选取健康成年SD大鼠[n=60,(250±30)g],随机分为5组:假手术组、休克组、休克复苏组、ω-6组、ω-3组。氯胺酮腹腔麻醉后,暴露右侧股动静脉并插管,诱导休克,使平均动脉压维持在35～40 mm Hg间,持续60 min。后利用回输1/2失血量的全血和Ringer液进行复苏,复苏过程持续30 min,平均动脉压维持在休克前的90%,ω-6组和ω-3组在复苏前30 min经尾静脉注射脂肪乳和鱼油(0.2 g/kg)。各组分别在复苏后30 min和4 h处死50%大鼠,取血清、肺标本。结果:ω-3组大鼠血清炎症及氧化应激指标、肺组织中炎症因子水平与假手术组无统计学差异,并明显低于其他3组;血清GSH在各组间无统计学差异。ω-3组肺组织病理评分及湿重/干重比明显低于休克组、休克复苏组及ω-6组。结论:复苏前给予ω-3PUFAs制剂能够明显抑制大鼠全身炎症反应,并减轻肺损伤程度。
OBJECTIVE: To study the inhibition of systemic inflammatory response and the protective effect on lung in rats after hemorrhagic shock and resuscitation by intravenous infusion of omega-3 polyunsaturated fatty acids before resuscitation. Methods: Healthy adult Sprague-Dawley rats were randomly divided into 5 groups: sham operation group, shock group, shock resuscitation group, omega-6 group and omega-3 group [n = 60, 250 ± 30] g. After intraperitoneal anesthesia, ketamine was exposed to the right femoral vein and intubated to induce shock. The mean arterial pressure was maintained at 35-40 mm Hg for 60 min. Resuscitation was performed using whole blood transfused with 1/2 of blood loss and Ringer’s solution for 30 min. The mean arterial pressure was maintained at 90% of the pre-shock level. The ω-6 and ω-3 groups were resuscitated 30 min before resuscitation Lateral injection of fat emulsion and fish oil (0.2 g / kg). In each group, 50% rats were sacrificed at 30 min and 4 h after resuscitation respectively, serum and lung samples were taken. Results: The levels of serum inflammation and oxidative stress in the omega-3 group were not significantly different from those in the sham operation group and significantly lower than those in the other three groups. Serum GSH levels were not significantly different among the three groups. The lung histopathological score and wet weight / dry weight ratio in the omega-3 group were significantly lower than those in the shock group, the shock resuscitation group and the omega-6 group. Conclusion: The administration of omega-3 PUFAs before resuscitation can significantly inhibit the systemic inflammatory response and reduce the degree of lung injury in rats.