论文部分内容阅读
这个简报叙述一个新的筛选抗癌药物的简便的离体细胞检查方法。这个方法是新的,是我们在进行小鼠艾氏腹水癌研究工作中摸索到的一个方法。运用这个方法,筛选那些直接作用于癌细胞的药物时极为有效。 筛选方法:——由小鼠腹水中取出新鲜腹水癌细胞悬液。用玻璃毛细管吸收癌细胞悬液,分别在玻璃载片的二处各滴一滴。再取药的20％水剂一小滴,满于一处癌细胞悬液中。载片上二处再分别滴上台氏生理盐水一小滴。等候20分钟,加伊红——台氏生理盐水。再等候2—3分钟,加上玻璃盖片,在显微镜下观察。死的癌细胞由于细胞液改变
This briefing describes a new, simple method for ex vivo cell screening of screening anticancer drugs. This method is new and one of the methods we have explored in the study of mouse Ehrlich ascites carcinoma. Using this method, it is extremely effective to screen for drugs that act directly on cancer cells. Screening method: - Remove fresh ascites cancer cell suspension from mouse ascites. A glass capillary was used to absorb the cancer cell suspension, and one drop was dropped on each of the glass slides. Take 20% of the drug solution as a small drop and fill it with a cancer cell suspension. Two drops of Tai’s normal saline were dropped on the slides. Wait 20 minutes, Jia Yihong - Taichung normal saline. Wait for 2-3 minutes, add a cover glass and observe under a microscope. Dead cancer cells due to changes in cytosol