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基于竞争双标记时间分辨荧光免疫分析技术,建立了一种新的同时测定猪肉组织中氯霉素(CAP)和莱克多巴胺(RAC)的方法.方法对CAP检出限和加标回收率分别为0.06ng/g和102%～121%(加标水平为0.1～5ng/gCAP),对RAC检出限和加标回收率分别为0.25ng/g和69.8%～85.8%(加标水平为1～10ng/gRAC).对18个猪肉样品的分析表明,本研究所建立的竞争双标记时间分辨荧光免疫分析法的测定结果与ELISA及GC-MS法一致,相关系数在0.92～0.98之间.
A new method for simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) in pork tissues was established based on competitive double-labeling time-resolved fluorescence immunoassay technique.RESULTS The detection limits and recoveries of CAP were The detection limits and recoveries of RAC were 0.25ng / g and 69.8% ~ 85.8% respectively at the concentrations of 0.06ng / g and 102% ~ 121% (spiked levels of 0.1 ~ 5ng / gCAP) ~ 10ng / gRAC) .The analysis of 18 pork samples showed that the competitive double-labeled time-resolved fluorescence immunoassay established in this study was consistent with that of ELISA and GC-MS, and the correlation coefficient was between 0.92 and 0.98.