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实验用雄性大鼠,混合接触500ppm 1,1,1-三氯乙烷和200ppm 三氯乙烯4天,每天6小时。第五天断头处死两只,其余分别继续染毒2或6小时后处死。然后迅速取出脑、肝、肺和肾周围的脂肪样品,于密闭的容器中称重,同时将血放入有肝素的试管中,盖紧管口。以4ml 甲苯-0.1M 磷酸钾缓冲液[7.2pH(V/V)]将组织(1.0g)做成匀浆,置于密封的试管中,在4℃下9,500g 离心10分钟。血液(1.0ml)用1ml 纯甲苯萃取,离心。用气-液色谱检测甲苯萃取液中的氯烃含量。并对上述样品进行各项生化指标的测定。
Experimental male rats were exposed to 500 ppm 1,1,1-trichloroethane and 200 ppm trichlorethylene for 4 days, 6 hours a day. On the fifth day, two were decapitated, and the rest were continued to be killed after 2 or 6 hours of exposure. Immediately remove the fat samples from the brain, liver, lungs and kidneys and weigh in a closed container. Place the blood in a test tube with heparin and cap the orifice. Tissue (1.0 g) was homogenized with 4 ml of toluene-0.1 M potassium phosphate buffer [7.2 pH (V / V)] and placed in a sealed tube and centrifuged at 9,500 g at 4 ° C for 10 minutes. Blood (1.0 ml) was extracted with 1 ml of pure toluene and centrifuged. Determination of Chlorohydrocarbons in Toluene Extracts by Gas Chromatography. And the above samples for the determination of the biochemical indicators.