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观测ＳＨ１对ＡＤＰ，ＡＡ，Ｃｏｌａｇｅｎ诱导的兔血小板聚集及ＴＸＢ２和６－ｋｅＴＯ－ＰＧＦＩα生成的影响。用比浊法测定了透骨草提取的单体生物碱结晶ＳＨ１体外对兔血小板聚集的影响，用放免法测定ＴＸＢ２和６－ｋｅＴＯ－ＰＧＦＩα的含量。结果：ＳＨ１０．８～３．０ｍｍｏｌ／Ｌ范围内明显抑制ＡＡ，ＡＤＰ，Ｃｏｌａｇｅｎ诱导的兔血小板聚集。ＳＨ１对三种诱导剂的最大抑制率分别为６２．１６％，４５．２５％，５３．６７％。大剂量组明显加快ＡＤＰ诱导的血小板聚集后的解聚速度，ＳＨ１显著延长Ｃｏｌａｇｅｎ的诱导起聚时间。其抑制作用有明显的量效关系，对ＡＡ诱导的ＴＸＢ２产生明显抑制作用，可使６－ｋｅＴＯ－ＰＧＦＩα的含量略有增加，但６－ｋｅＴＯ－ＰＧＦＩα／ＴＸＢ２比值显著增大
To observe the effect of SH1 on platelet aggregation induced by ADP, AA and Colagen and the generation of TXB2 and 6-keTO-PGFIα. The turbidimetric method was used to determine the effect of the extracellular alkaloid extracted from Septumria divaricata on platelet aggregation in vitro. The content of TXB2 and 6-keTO-PGFIα were determined by radioimmunoassay. Results: Rabbit platelet aggregation induced by AA, ADP and Colagen was significantly inhibited in SH10.8 ~ 3.0mmol / L. The maximum inhibitory rates of SH1 to the three inducing agents were 62.16%, 45.25% and 53.67%, respectively. The high-dose group significantly accelerated the rate of ADP-induced platelet aggregation, and SH1 significantly prolonged Colagen-induced polymerization. The inhibitory effect was significantly dose-effect relationship, which could inhibit the TXB2 induced by AA and increase the content of 6-keTO-PGFIα slightly, but the ratio of 6-keTO-PGFIα / TXB2 was significantly increased