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增殖性细胞核抗原(PCNA)系一高度恒定的36KD核酸蛋白和DNA合成直接相关.文章就PCNA免疫反应性和DNA流式细胞分析与组织病理检查结果进行了对比.材料与方法:选取淋巴结阴性经根治手术治疗,未接受放疗、化疗和内分泌疗法的40例周围型肺小细胞肺癌(NSCLC)的石腊切片,脱腊后采用免疫球蛋白G2a单克隆抗体PC10进行免疫组化染色,根据染色阳性的细胞比例,划分PCNA免疫反应性程度,低度为1%~25%,中度为26%~75%,高度为76%~100%.有丝分裂指数(MC)根据光镜检查和病理特征.
Proliferating cell nuclear antigen (PCNA) is a highly constant 36KD nucleic acid protein that is directly related to DNA synthesis. The article compares PCNA immunoreactivity with DNA flow cytometric analysis and histopathological examination. Materials and Methods: Select lymph node negative Radical surgery, 40 cases of peripheral lung cancer (NSCLC) without radiotherapy, chemotherapy, and endocrine therapy were smeared with paraffin and immunohistochemically stained with immunoglobulin G2a monoclonal antibody PC10 according to staining positive The proportion of cells divided PCNA immunoreactivity to a low degree of 1% to 25%, moderate to 26% to 75%, and a height of 76% to 100%. The mitotic index (MC) was based on light microscopy and pathological features.