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镉试剂2B-曲拉通 X-100-Zn 三元分光光度系测Zn(Ⅱ)的最佳条件如下:25ml 最终容积中含 Zn(Ⅱ)≤12μg,5%m/V 四硼酸钠溶液(pH9.2)3.0±0.5ml、2%V/V 曲拉通溶液1.5ml、0.04%镉试剂2B 乙醇溶液2.5(2.0～3.5)ml。pH 值宜控制在8.5～9.8,曲拉通 X-100溶液的用量可在0.5～4.0ml 范围内变
The optimal conditions for the determination of Zn (Ⅱ) by the cadmium reagent 2B-Triton X-100-Zn ternary spectrophotometric system are as follows: The final volume of 25ml contains Zn (Ⅱ) ≤12μg, 5% m / pH 9.2) 3.0 ± 0.5ml, 2% V / V triptolide solution 1.5ml, 0.04% cadmium reagent 2B ethanol solution 2.5 (2.0 ~ 3.5) ml. pH value should be controlled at 8.5 ~ 9.8, the amount of Triton X-100 solution can be within the range of 0.5 ~ 4.0ml