细胞融合是近年发展起来的微生物育种新技术,是用两亲株去壁后的原生质体进行融合而达到基因重组目的。这一方法的最大优点是能克服远缘杂交的困难,从而可望获得全新的杂种菌株。当前,国内外生物工作者运用这一手段在创造动、植物及微生物(主要是细菌、霉菌及酵母菌等)新种中均有较多成功的事例,但在食用菌方面还极少见有报导。进行原生质体融合的先决条件是必须有溶解细胞的酶。我们经多年试验,己制备出一种能溶解食用菌细胞壁的酶制剂,称溶壁酶。通过几年试用,证实该酶能酶解多种食用菌细胞壁,获得有生活力的原生质体。我们用溶壁酶酶解了草菇、双孢磨菇、黑木 Cell fusion is a new technology of microbial breeding which has been developed in recent years. It is the purpose of gene recombination by protoplast fusion with amphipathic strain. The biggest advantage of this method is that it can overcome the difficulties of distant hybridization and hopefully obtain a brand new hybrid strain. At present, biologists at home and abroad use this method to create more success stories in new species of animals, plants and microorganisms (mainly bacteria, mold and yeast), but rare in edible fungi Reported. A prerequisite for protoplast fusion is that there must be enzymes that lyse the cells. After years of testing, we have prepared an enzyme preparation that dissolves the cell walls of edible fungi, called lywallzyme. After several years of trial, confirmed that the enzyme can digest a variety of edible fungi cell wall, to obtain viable protoplasts. We use Lysozyme enzymatic hydrolysis of mushrooms, spores mushroom, black wood