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目的:研究参麦注射液对人p53缺失Hep3B肝癌细胞增殖及凋亡的影响。方法:将细胞随机分为对照组及不同浓度参麦注射液(40μL/m L、80μL/m L、160μL/m L和320μL/m L)处理组,孵育24 h后,采用MTT比色法、流式细胞术、彗星实验和Western bloting法分别检测参麦注射液对Hep3B细胞活力、胞内ROS水平、细胞凋亡率、DNA损伤及胞浆中细胞色素C的影响。结果:参麦注射液可以引起Hep3B细胞存活率下降,胞内ROS水平明显升高、DNA损伤加重、胞浆中细胞色素C表达及细胞凋亡率增加。结论:参麦注射液可抑制Hep3B细胞增殖,且可能通过胞内ROS水平升高诱导DNA损伤加重,并可能诱导了线粒体依赖的细胞凋亡。
Objective: To study the effect of Shenmai injection on proliferation and apoptosis of Hep3B hepatoma cells with human p53 deletion. Methods: The cells were randomly divided into control group and different concentrations of Shenmai injection (40μL / m L, 80μL / m L, 160μL / m L and 320μL / m L) treatment group, incubated for 24 h, using MTT colorimetric , Flow cytometry, comet assay and Western blotting method were used to detect the effect of Shenmai injection on Hep3B cell viability, intracellular ROS level, apoptotic rate, DNA damage and cytosolic cytochrome C in Hep3B cells. Results: Shenmai injection could decrease the survival rate of Hep3B cells, increase the intracellular ROS levels, aggravate DNA damage and increase the expression of cytochrome C in cytoplasm and the rate of apoptosis. Conclusion: Shenmai injection can inhibit the proliferation of Hep3B cells and induce the DNA damage possibly through the increase of intracellular ROS levels, and may induce mitochondria-dependent apoptosis.