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目的:建立同时测定芩翘口服液中黄芩苷、连翘苷含量的方法。方法:色谱柱为Inertsil ODS-SP(250 mm×4.6 mm,5μm);流动相:磷酸盐缓冲液(取磷酸二氢铵11.5 g,加适量水使溶解,加磷酸1 ml,用水稀释至1 000 ml)-乙腈(75∶25);流速:1.0 ml·min-1;柱温:25℃;检测波长:278 nm。结果:黄芩苷线性范围为0.203~4.064μg,r=0.999 8,平均回收率100.9%,RSD为1.7%(n=5);连翘苷线性范围为0.182~3.648μg,r=0.999 7,平均回收率100.5%,RSD为1.4%(n=5)。结论:该方法简便,准确,重复性好,可用于芩翘口服液中黄芩苷、连翘苷含量的测定。
Objective: To establish a method for simultaneous determination of baicalin and forsythin in QinQian Oral Liquid. Methods: The column was Inertsil ODS-SP (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of phosphate buffer (11.5 g ammonium dihydrogen phosphate, dissolved with appropriate amount of water, diluted to 1 with phosphoric acid 000 ml) -acetonitrile (75:25); flow rate: 1.0 ml · min-1; column temperature: 25 ° C .; detection wavelength: 278 nm. Results: The linear range of baicalin was 0.203-4.064μg, r = 0.999 8, the average recovery was 100.9% with RSD 1.7% (n = 5). The linear range of forsythin was 0.182 ~ 3.648μg, r = 0.999 7, The recovery was 100.5% and the RSD was 1.4% (n = 5). Conclusion: The method is simple, accurate and reproducible. It can be used for the determination of baicalin and forsythin in QinQian Oral Liquid.