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目的检测肾细胞癌组织及相应癌旁正常组织中miR-7的表达,分析18达与临床病理特征之间的关系,为进一步研究miR-7在肾癌的发生发展中作用奠定基础。方法收集48例经手术切除的配对肾癌及癌旁组织标本,提取组织中总RNA,经逆转录获得cDNA,采用实时荧光定量PCR(RT-qPCR)方法检测标本中miR-7表达量,并通过统计学软件分析其表达量与患者临床病理特征之间的相关性。结果与相应癌旁组织相比,肾癌组织中miR-7表达量明显升高,其中34例(70.8%)肾癌标本miR-7表达上调,14例(29.2%)表达下调,差异有统计学意义(P<0.05)。肾细胞癌中miR-7表达上调与患者年龄、性别、肾癌组织类型、TNM分期、AJCC临床分期无相关性(P>0.05)。结论 miR-7在肾癌组织中明显高表达,提示其可能与肾癌的发生和发展相关,可能成为肾癌早期诊断、治疗乃至预后判断的新的生物标记物。
Objective To detect the expression of miR-7 in renal cell carcinoma tissues and adjacent normal tissues, and to analyze the relationship between miR-7 and clinicopathological features, so as to lay the foundation for further study on the role of miR-7 in the carcinogenesis and progression of renal cell carcinoma. Methods 48 cases of matched matched renal cell carcinoma and adjacent normal tissues were collected. Total RNA was extracted from tissue and reverse transcribed to obtain cDNA. The expression of miR-7 was detected by real-time fluorescence quantitative PCR (RT-qPCR) Statistical software was used to analyze the correlation between the expression level and the clinicopathological features of patients. Results The expression of miR-7 in renal cell carcinoma tissues was significantly higher than that in adjacent non-cancerous tissues. MiR-7 expression was up-regulated in 34 (70.8%) of renal cell carcinoma specimens and down-regulated in 14 (29.2%) cases Significance (P <0.05). The upregulation of miR-7 in renal cell carcinoma had no correlation with the age, sex, type of renal cell carcinoma, TNM staging, clinical stage of AJCC (P> 0.05). Conclusion The high expression of miR-7 in renal cell carcinoma suggests that miR-7 may be associated with the occurrence and development of renal cell carcinoma, which may be a new biomarker for the early diagnosis, treatment and prognosis of renal cell carcinoma.