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目的观察骨质疏松症骨髓白细胞介素-6(IL-6)受体(IL-6R)亚单位gp80和gp130基因表达的变化,探讨IL-6R复合系统在雌激素缺乏所致骨质疏松中的作用。方法12周龄雌性SD大鼠20只犤体重(200±20)g犦随机分为骨质疏松组(10只)和空白对照组(10只)。分别于造模后0、12周,测定大鼠骨髓gp80和gp130mRNA水平。结果gp130mRNA水平(pg/μgtotalRNA):术后0、12周空白对照组无明显变化犤(80±3)和(82±4),t=0.85,P>0.05犦,骨质疏松组明显增加犤(80±3)和(290±40),t=6.46,P<0.01犦。gp80mRNA水平(pg/μgtotalRNA):术后0、12周空白对照组无明显变化增加犤(85.4±2.7)和(85.6±2.8),t=0.35,P>0.05犦,骨质疏松组明显增加犤(85.2±2.7)和(210±40),t=6.32,P<0.01犦。结论卵巢切除所致骨质疏松大鼠骨髓IL-6R亚单位gp80和gp130基因表达增加。
Objective To observe the changes of gp80 and gp130 gene expressions in the osteoporosis patients with interleukin-6 (IL-6) receptor (IL-6R) subunits, and to explore the role of IL-6R in osteoporosis induced by estrogen deficiency Role. Methods Twenty female SD rats aged 12 weeks were randomly divided into osteoporosis group (n = 10) and blank control group (n = 10). The levels of gp80 and gp130 mRNA in bone marrow of rats were measured at 0 and 12 weeks after model establishment. Results The levels of gp130 mRNA (pg / μgtotalRNA) in the control group at 0 and 12 weeks after operation were not significantly different from those in the control group (± 80 ± 3 and 82 ± 4, t = 0.85, P> 0.05 犦) (80 ± 3) and (290 ± 40), t = 6.46, P <0.01 犦. The level of gp80 mRNA (pg / μgtotalRNA) in the control group increased significantly (.4 85.4 ± 2.7 vs 85.6 ± 2.8, t = 0.35, P> 0.05 犦) at 0 and 12 weeks after operation, but was significantly increased in the osteoporosis group (85.2 ± 2.7) and (210 ± 40), t = 6.32, P <0.01 犦. Conclusions The expression of gp80 and gp130 genes of IL-6R subunits in osteoporosis rats after ovariectomy is increased.