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[目的]本研究旨在明确无鼓膜发音器的华蝉族(Sinosenini)昆虫在蝉总科(Cicadoidea)的系统发育地位.[方法]依据在陕西宁陕采集的合哑蝉Karenia caelatata成虫标本,对华蝉族的合哑蝉K.caelatata线粒体基因组进行测序、注释和生物信息学分析;并与蝉总科其他类群的线粒体基因组进行了比较,然后利用最大似然法(ML)和贝叶斯法(BI)分别构建了蝉总科分子系统发育树.[结果]合哑蝉线粒体基因组长14 960 bp(GenBank登录号:MN922304),其基因组成、蛋白编码基因的核苷酸组成和密码子使用等,与蝉总科其他类群具相似特征.核苷酸多样性分析表明,atp8,rnnad6和nad2为易变基因,而cox1比较保守.非同义替换率和同义替换率比表明,蝉总科昆虫线粒体基因组进化处于高水平的纯化选择下.系统发育分析结果支持蝉次目(Cicadomorpha)的单系性,该次目3个总科的关系为:角蝉总科Membraciodea+(蝉总科Cicadoidea+沫蝉总科Cercopodidea).无鼓膜发音器的哑蝉属与蝉亚科(Cicadinae)的蜩蝉族(Dundubiini)相关类群聚在一起,且与寒蝉属Meimunaa关系最近;黑蝉族(Cicadatrini)的草蝉属Mogannia和音蝉属Vagitanus则与姬蝉亚科(Cicadettinae)相关类群聚在一起;日宁蝉属Yezoterpnosia并非一个单系群.[结论]华蝉族应从姬蝉亚科转移至蝉亚科并与蜩蝉族(Dundubiini)合并,而黑蝉族应从蝉亚科转移至姬蝉亚科.研究结果为进一步解析具有不同发声机制的蝉科昆虫系统演化提供了新信息.“,”[Aim]The aim of this study is to clarify the phylogenetic status of the tribe Sinosenini in Cicadoidea,in which the cicadas have no timbal organs.[Methods]Based on adult specimens of Karenia caelatata of Sinosenini collected in Ningshan,Shaanxi,northwestern China,the mitochondrial genome of K.caelatata was sequenced,annotated,bioinformatically analyzed,and compared with the mitochondrial genomes of other taxa of Cicadoidea.Molecular phylogenetic trees for Cicadoidea were constructed using both maximum likelihood(ML)method and Bayesian inference(BI)method.[Results]The mitochondrial genome of K.caelatata(GenBank accession number:MN922304)is 14 960 bp in length,and the gene organization,nucleotide composition and codon usage of protein-coding genes display similar structural characteristics to those of other taxa of Cicadoidea.Analysis of nucleotide diversity reveals that genes atp8,nad6 and nad2 are highly variable,while cox 1 is more conserved.Ratios of nonsynonymous and synonymous substitution rates indicate that the evolution of mitochondrial genomes in Cicadoidea is under a high-level purifying selection.Results of phylogenetic analyses support the monophyly of Cicadomorpha,revealing the relationship of the three superfamilies of this infraorder as(Membraciodea+(Cicadoidea+Cercopodidea)).Karenia is clustered with representatives of the tribe Dundubiini in the subfamily Cicadinae and most closely related to the genus Meimuna.Mogannia and Vagitanus,representatives of Cicadatrini,are clustered with the members of Cicadettinae.Yezoterpnosia is not a monophyletic group.[Conclusion]Sinosenini should be transferred from Cicadettinae to Cicadinae and merged with Dundubiini,while Cicadatrini should be transferred from Cicadinae to Cicadettinae.Our results provide new information for future study of evolution of cicadas with different sound-producing mechanisms.