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目的:探讨人类白细胞抗原HLA-DQ-A1、DQB1、DPA1基因多态性与先兆子痫发病的关系。方法:采用序列特异性引物技术(PCRSSP)对46例先兆子痫患者和105例正常孕妇及其新生儿进行HLA-DQ-DPA1等位基因分型。结果:所有标本共检出11种HLADQA1基因表型、16种HLADQB1基因表型、6种HLADPA1基因表型。先兆子痫患者HLA-DQ-B10301基因频率高于正常孕妇,差异有显著性(Pc=0.032,RR=2.43,AR=0.30),其余各基因表型频率两组比较差异均无显著性。结论:HLADQB10301基因可能是一种先兆子痫发病的易感基因。
Objective: To investigate the relationship between human leukocyte antigen HLA-DQ-A1, DQB1, DPA1 gene polymorphisms and preeclampsia. Methods: HLA-DQ-DPA1 alleles were genotyped in 46 patients with preeclampsia and 105 normal pregnant women and their newborns by using sequence-specific primers (PCRSSP). RESULTS: Eleven HLADQA1 phenotypes, 16 HLADQB1 phenotypes and 6 HLADPA1 phenotypes were detected in all specimens. The frequency of HLA-DQ-B10301 gene in preeclampsia patients was significantly higher than that in normal pregnant women (Pc = 0.032, RR = 2.43, AR = 0.30). There was no significant difference in the frequencies of other phenotypes between the two groups. Conclusion: HLADQB10301 gene may be a susceptibility gene for the development of preeclampsia.