论文部分内容阅读
目的探讨芍药苷对脑外伤大鼠模型行为学及下丘脑-垂体-肾上腺(HPA)轴的影响。方法将45只大鼠随机分为假手术组(n=15)、脑外伤组(n=15)、芍药苷组(n=15)。按照改良Feeney法制备脑外伤大鼠模型。芍药苷组术后3 h给予芍药苷2.0 mg/kg腹腔注射,假手术组不进行任何处理,脑外伤组注射等量生理盐水,2次/d。24 h后处死。采用定位航行试验和空间探索试验评价行为学,采用酶联免疫法测定下丘脑CRH和血清CS含量。结果与假手术组比较,脑外伤组、芍药苷组第1周和第2周大鼠逃避潜伏期明显延长(P<0.05),而芍药苷组第4周大鼠逃避潜伏期比较无统计学差异(P>0.05);与脑外伤组比较,芍药苷第1、第2、第4周大鼠逃避潜伏期明显降低(P<0.05);与假手术组比较,脑外伤组、芍药苷组第1、第2、第4周大鼠游泳时间百分比明显减少(P<0.05);与脑外伤组比较,芍药苷组第1、第2、第4周大鼠游泳时间百分比明显增加(P<0.05);与假手术组比较,脑外伤组、芍药苷组下丘脑CRH及血清CS含量明显上升(P<0.05);与脑外伤组比较,芍药苷组下丘脑CRH及血清CS含量明显降低(P<0.05)。结论芍药苷可明显改善脑外伤大鼠模型行为学,且对脑外伤大鼠模型HPA轴具有一定的调节作用。
Objective To investigate the effects of paeoniflorin on the behavior and the hypothalamic-pituitary-adrenal (HPA) axis in a rat model of traumatic brain injury. Methods Forty five rats were randomly divided into sham operation group (n = 15), traumatic brain injury group (n = 15) and paeoniflorin group (n = 15). The rat model of traumatic brain injury was prepared according to the modified Feeney method. The paeoniflorin group was given intraperitoneal injection of paeoniflorin 2.0 mg / kg 3 h after operation. The rats in the sham operation group were not given any treatment. The traumatic brain injury group was injected with normal saline twice a day. After 24 h sacrificed. The navigation behavior and space exploration test were used to evaluate the behavior. The contents of CRH and serum CS in the hypothalamus were determined by enzyme-linked immunosorbent assay. Results Compared with sham operation group, the escape latency of rats in traumatic brain injury group and paeoniflorin group was significantly prolonged at 1 week and 2 weeks (P <0.05), while there was no significant difference in the escape latency of 4 weeks in paeoniflorin group P <0.05). Compared with the traumatic brain injury group, the escape latency of paeoniflorin in the first, second and fourth weeks was significantly decreased (P <0.05). Compared with the sham-operated group, Compared with the traumatic brain injury group, the percentage of swimming time in the first, second and fourth weeks of paeoniflorin group was significantly increased (P <0.05); Compared with the sham group, the levels of CRH and serum CS in the traumatic brain injury group and the paeoniflorin group increased significantly (P <0.05); compared with the traumatic brain injury group, the content of CRH and serum CS in the hypothalamus of the paeoniflorin group decreased significantly (P <0.05 ). Conclusion Paeoniflorin can significantly improve the behavioral model in rats with traumatic brain injury and regulate the HPA axis in rats with traumatic brain injury.