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目的 研究天然和破壁松花粉中锌的生物利用度。方法 采用高速气流粉碎技术 ,将松花粉干法破壁 ;并使用同位素稀释技术 ,对天然和破壁松花粉中锌的生物利用度进行了研究。雄性生长中 SD大鼠 30只 ,平均体重 85g,肌注同位素65Zn(1 70 k Bq65Zn溶于 75μl0 .88%Na Cl生理盐水中 )。随机分为三组 ,每组 1 0只。组 1 (对照组 )食用基础饲料 ;组 2、组 3食用含 6%破壁和天然松花粉制成的饲料 ,三者含锌量均为 7μg/g。 1 7d实验期间给每只大鼠 9.4g/d食物 ,定量收集粪、尿 ,并使用同位素稀释技术测定内源性锌的排出和锌的真实吸收量。结果 实验结束时各组大鼠间体重和血浆锌未见差异 ,食用含松花粉饲料的大鼠血液学参数略高于对照组。天然和破壁松花粉粪锌排泄分别增加了 53%和 38%。这是由于食用含松花粉饲料的两组大鼠内源性粪锌的排泄上升了 64%所致。在这一情况下食用含天然松花粉饲料组的大鼠锌生物利用度较对照组(96% )低 ,为 94.4% ;而食用含破壁松花粉饲料组锌的生物利用度没有改变 (96% )。结论 松花粉破壁后锌生物利用度有所上升 ,但与天然松花粉本来已是很高的锌生物利用度水平相比幅度很小
Objective To study the bioavailability of zinc in natural and broken pollen. Methods The high-speed air-jet milling technique was used to dry the pine pollen. The isotope dilution technique was used to study the bioavailability of zinc in natural and broken pollen. Thirty male Sprague-Dawley rats weighing 85 g were injected intramuscularly with isotope 65Zn (1 70 k Bq65Zn in 75 μl of 0.88% NaCl saline). Randomly divided into three groups, each group of 10. Group 1 (control group) consumed basic feed; Group 2 and Group 3 consumed feed containing 6% broken and natural pine pollen, both of which contained 7μg / g zinc. During each experiment, 9.4 g / d of food was administered to each rat during the experiment, feces and urine were collected quantitatively, and the excretion of endogenous zinc and the true absorption of zinc were determined using isotope dilution technique. Results At the end of the experiment, there was no difference in body weight and plasma zinc among rats in each group. The hematological parameters of rats with pine pollen diet were slightly higher than those of the control group. Natural and broken pine pollen excretion of zinc increased by 53% and 38%. This is due to the 64% increase in excretion of endogenous fecal zinc in both groups of rats with pine pollen feed. In this case, the bioavailability of zinc in the diet containing natural pine pollen was lower than that in the control group (96%), 94.4%, while the bioavailability of zinc in the diet containing broken pine pollen did not change (96 %). Conclusion The bioavailability of zinc increased after the pollen wall was broken, but it was small compared with the already high zinc bioavailability of natural pine pollen