论文部分内容阅读
在总胆管阻塞病人中,引起死亡最主要的原因为脓毒症。脓毒症的多见可能与网状内皮系统功能减弱有关,其中主要是枯否氏细胞的吞噬作用减弱。本文作者用大鼠模型作实验观察持续胆道阻塞是否影响血中细菌清除,并评价各器官对存活细菌的羁留作用。方法:100～125g的雄性大鼠48只,分成三组,在醚麻下剖腹。第Ⅰ组10只为对照组,仅翻动十二指肠及脾脏;第Ⅱ组28只,作总胆管结扎及切断(CDL);第Ⅲ组10只,作脾切除术。手术后3周,以10~9个~(35)硫标记的大肠杆菌静脉注入各鼠。注射后1、2、3、5、8及10分钟采血测定细菌清除率及生化指标。注射10分钟后将肝、脾、肾及肺切除秤重,采样测定各器官中的细菌分
Among the patients with total cholangiocarcinoma, the most common cause of death is sepsis. The more common sepsis may be related to the reticuloendothelial dysfunction, in which the phagocytosis of Kupffer cells is mainly weakened. The authors used a rat model to experimentally observe whether persistent biliary obstruction affects bacterial clearance in blood and evaluate the detention of surviving bacteria in various organs. Methods: Forty-eight male rats (100 ~ 125g) were divided into three groups and were given cesarean section under ether anesthesia. Group I only 10 as the control group, only to turn the duodenum and spleen; Group II 28, for the common bile duct ligation and cutting (CDL); the third group of 10, for splenectomy. Three weeks after surgery, 10 ~ 9 ~ (35) sulfur-labeled Escherichia coli were intravenously injected into each mouse. Blood samples were taken at 1, 2, 3, 5, 8 and 10 minutes after injection to determine bacterial clearance rate and biochemical indexes. After 10 minutes of injection, the liver, spleen, kidney and lung were resected and weighed to determine the bacterial fraction in each organ