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目的了解苯和甲醛吸入染毒对小鼠血清、肺、肝脏的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的活力影响。方法将昆明种雄性小鼠40只,随机分为4组,分别为对照组、甲醛组(3 mg/m3)、苯组(2 000 mg/m3)和联合组(甲醛3 mg/m3+苯2 000 mg/m3)。每天给予4 h吸入染毒,每周5 d,连续5周。测定动物体重、肺脏和肝脏的脏器系数;测定血清、肺、肝脏的SOD、GSH-Px和MDA水平。结果经过5周染毒,苯组和联合染毒组动物平均体重低于对照组(P<0.05),3个组动物的血清中SOD水平明显低于对照组(P<0.01),苯组和联合染毒组肺脏系数明显低于对照组(P<0.01),苯组和联合组肺脏SOD、GSH-Px水平高于对照组(P<0.05),联合组的肺脏MDA水平较对照组明显升高(P<0.01);3个染毒组的肝脏系数均明显低于对照组(P<0.01),苯组和联合组肝脏SOD和MDA水平高于对照组(P<0.01),甲醛组和联合组的GSH-Px的水平高于对照组(P<0.01)。结论苯和甲醛吸入后可能造成血清中SOD水平下降,引起肺、肝脏的SOD和GSH-Px的活力增高,苯和甲醛的联合染毒造成的过氧化损伤大于单独染毒。
Objective To investigate the effects of inhalation of benzene and formaldehyde on the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in serum, lung and liver of mice. Methods Forty Kunming male mice were randomly divided into 4 groups: control group, formaldehyde group (3 mg / m3), benzene group (2 000 mg / m3) and combination group 000 mg / m3). 4 h daily inhalation exposure, 5 d per week for 5 weeks. The body weight, lung and liver organ coefficients were measured. The levels of SOD, GSH-Px and MDA in serum, lung and liver were measured. Results After 5 weeks exposure, the average body weight of the animals in the benzene group and the combined group was lower than that in the control group (P <0.05). The levels of SOD in the three groups were significantly lower than those in the control group (P <0.01) The lung coefficient of the combined exposure group was significantly lower than that of the control group (P <0.01). The levels of SOD and GSH-Px in the lungs of the benzene group and the combined group were higher than those of the control group (P <0.05) (P <0.01). The liver coefficient of the three exposure groups was significantly lower than that of the control group (P <0.01). The levels of SOD and MDA in the liver of the benzene group and the combined group were higher than those in the control group (P <0.01) The combined group of GSH-Px levels higher than the control group (P <0.01). Conclusion The inhalation of benzene and formaldehyde may cause the decrease of SOD in serum and cause the increase of SOD and GSH-Px in lung and liver, and the peroxidation damage caused by the combination of benzene and formaldehyde is higher than that of single exposure.