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[目的]研究海尔福对慢性氟中毒小鼠的治疗效果,为防治地方病氟中毒提供有效的药物。[方法]将30只健康小鼠随机分为3组,对照组、染毒组[100 mg/L氟化钠(NaF)]及治疗组(100 mg/L NaF+海尔福口服液)进行治疗。治疗后,通过测定血、股骨、粪便的氟含量,测定并分析肾组织上清液中脂质过氧化代谢产物:丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷光苷肽过氧化物酶(GSH-Px)的活性,以观察海尔福对氟中毒小鼠的治疗效果。[结果]血氟、骨氟含量:均以染毒组最高,分别是(5.68±1.42)mol/L、(2.97±1.92)mol/L;治疗组分别是(3.53±0.68)mol/L、(1.19±0.76)mol/L。染毒组与治疗组比较,差异有统计学意义(P<0.01);粪氟含量以治疗组最高(0.76±0.18)mol/L,与其他2组比较差异有统计学意义(P<0.01);肾组织中MDA含量、GSH-Px和SOD活性;中毒组[分别是(146.18±17.23)μmol/L、(100.23±16.34)×103 U/L、(92.35±7.83)U]与对照组[分别是(9.05±2.62)μmol/L、(379.08±10.02)×103 U/L、(342.04±13.65)U]及治疗组[分别是(72.46±5.83)μmol/L、(340.52±11.06)×103 U/L、(261.87±11.46)U]比较,差异均有统计学意义(P<0.01)。[结论]海尔福可能通过促进氟的排泄,在一定程度上恢复SOD、GSH-Px的活性,对慢性氟中毒小鼠有一定的治疗作用。
[Objective] To study the therapeutic effect of Haierfu on mice with chronic fluorosis and to provide an effective drug for the prevention and treatment of endemic fluorosis. [Method] Thirty healthy mice were randomly divided into three groups. The control group, the poisoned group [100 mg / L sodium fluoride (NaF)] and the treatment group (100 mg / L NaF + Haier Fu oral solution) were treated. After treatment, the contents of lipid peroxidation metabolites such as malondialdehyde (MDA), superoxide dismutase (SOD), glutathione in renal supernatant were measured and analyzed by measuring the content of fluoride in blood, femur and feces. Peroxidase (GSH-Px) activity in order to observe the therapeutic effect of Haifu Fu on mice with fluorosis. [Results] The contents of blood fluoride and bone fluoride in the treatment group were the highest (5.68 ± 1.42) mol / L and (2.97 ± 1.92) mol / L respectively, and those in the treatment group were (3.53 ± 0.68) mol / L and (1.19 ± 0.76) mol / L. Compared with the treatment group, the difference was statistically significant (P <0.01); the content of fecal fluoride in the treatment group was the highest (0.76 ± 0.18) mol / L, with statistical significance (P <0.01) ; The content of MDA and the activity of GSH-Px and SOD in kidney tissue were significantly higher than those in the control group [(146.18 ± 17.23) μmol / L, (100.23 ± 16.34) × 103 U / L and (92.35 ± 7.83) U] (37.05 ± 2.62) μmol / L, (379.08 ± 10.02) × 103 U / L, (342.04 ± 13.65) U] and the treatment group [(72.46 ± 5.83) μmol / L, (340.52 ± 11.06) × 103 U / L, (261.87 ± 11.46) U], the differences were statistically significant (P <0.01). [Conclusion] Haier Fu may restore the activity of SOD and GSH-Px to a certain extent by promoting the excretion of fluoride, and may have a certain therapeutic effect on mice with chronic fluorosis.