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目的 :研究鸟氨酸脱羧酶 (ODC)基因在结直肠癌中的表达 ,探讨其在大肠癌形成中的作用。方法 :建立测定ODC基因mRNA表达的逆转录多聚酶链反应 (RT PCR)最适条件 ,在半定量水平测定 31例结直肠癌的癌旁正常组织和癌组织中的ODCmRNA表达的相对水平 ,观察其与临床病理学指标的关系。结果 :31例结直肠癌中ODC基因表达较癌旁正常组织高 ,差异显著 (P <0 .0 1) ,ODC基因表达在性别、病理类型之间差异无显著意义 (P >0 .0 5 ) ,在Dukes分期之间差异有显著意义 (P =0 .0 12 )。结论 :ODC在大肠癌组织中表达增高并与恶性程度呈正相关 ,提示ODC表达增加与大肠癌发生、浸润及转移有关 ,为大肠癌病因及诊断和治疗方法研究提供了实验依据。
OBJECTIVE: To study the expression of ornithine decarboxylase (ODC) gene in colorectal cancer and to explore its role in the formation of colorectal cancer. Methods: The optimum condition of reverse transcription polymerase chain reaction (RT PCR) for determining ODC gene mRNA expression was established. The relative level of ODC mRNA expression in normal tissues and cancerous tissues of 31 cases of colorectal cancer was measured at semi-quantitative level. And clinicopathological parameters of the relationship. Results: The ODC gene expression was significantly higher in colorectal cancer tissues than in adjacent normal tissues (P <0.01). There was no significant difference in ODC gene expression between sex and pathological types (P> 0.05) ), There was a significant difference between Dukes staging (P = .012). CONCLUSION: The increased expression of ODC in colorectal cancer tissues is positively correlated with the degree of malignancy. It suggests that the increased expression of ODC is associated with the occurrence, invasion and metastasis of colorectal cancer. It provides an experimental basis for the etiology, diagnosis and treatment of colorectal cancer.