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目的建立一种新的移植肾缺血再灌注损伤(IRI)动物实验模型并寻找一种新的移植肾保护方法。方法40只兔随机分为A组(生理盐水对照组)、B组(单纯灌注液组)、C组[环孢素A(CsA)+灌注液组,CsA浓度为0.01‰,W/V]、D组(CsA+灌注液组,CsA浓度为0.03‰,W/V)。用套管针穿刺左肾动、静脉,灌注肾脏。原位低温保存2h后,切除右肾并开放左肾血流使左肾复灌。于术后6、24h分别取血标本,24h后取左肾标本,测定血尿素氮(BUN)、血肌酐(Cr),TUNEL法检测分析左肾小管上皮细胞凋亡率,免疫组织化学法测定左肾组织HSP70的表达。结果D组6、24hBUN为(8.60±0.25)mmol/L、(7.99±0.14)mmol/L;Cr为(79.90±1.37)μmol/L、(78.98±1.66)μmol/L,细胞凋亡指数为0.075±0.035,明显低于其他各组;HSP70表达为(0.211±0.025)μm2,明显高于其他各组。结论本模型能客观反映移植肾缺血再灌注损伤状态,0.03‰CsA复合肾灌注液能明显降低移植肾缺血再灌注损伤。
OBJECTIVE: To establish a new experimental animal model of renal ischemia-reperfusion injury (IRI) and to find a new method of renal allograft protection. Methods Forty rabbits were randomly divided into three groups: A group (normal saline control group), B group (simple perfusion group), C group (CsA + perfusion group, CsA concentration was 0.01 ‰, W / V] , Group D (CsA + perfusion group, CsA concentration of 0.03 ‰, W / V). Thoracotomy with left renal artery and vein, perfusion of the kidneys. In situ cryogenic preservation 2h, removal of the right kidney and left renal blood flow left renal reperfusion. The blood samples were taken at 6 and 24 h after operation, and the left renal samples were taken after 24 h. The levels of BUN, Cr and TUNEL in the left renal tubular epithelial cells were detected by immunohistochemistry Left kidney tissue HSP70 expression. Results The 6 and 24 h BUN in group D were (8.60 ± 0.25) mmol / L and (7.99 ± 0.14) mmol / L respectively, and the Cr values were (79.90 ± 1.37) μmol / L and 0.075 ± 0.035, significantly lower than other groups; HSP70 expression was (0.211 ± 0.025) μm2, which was significantly higher than other groups. Conclusion This model can objectively reflect the status of ischemia-reperfusion injury in renal allografts. 0.03? 000? CsA combined with renal perfusion can significantly reduce the renal ischemia - reperfusion injury.