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目的研究瓦斯爆炸伤肺组织中核因子kB(NF-kB)及细胞间黏附分子-1(ICAM-1)的表达及二者之间关系。方法建立瓦斯爆炸伤大鼠模型,用地高辛标记NF-kB cDNA探针检测肺组织NF-kB mRNA的表达,用免疫组化SP法检测肺组织NF-kB及ICAM-1的表达。结果瓦斯爆炸伤后各实验组大鼠肺组织中NF-kB mRNA及其蛋白表达、ICAM-1的蛋白表达均较对照组明显增多,在伤后2 h增多最明显。肺组织病理学检查显示,瓦斯爆炸伤后,部分气管黏膜上皮脱落、管壁明显充血、水肿、中性粒细胞浸润；肺泡出血、水肿、大量炎细胞浸润。电镜示Ⅱ型肺泡上皮细胞变性脱落,改变明显。结论瓦斯爆炸伤致NF-kB活化,并促进ICAM-1的表达,与急性肺损伤关系密切。
Objective To study the expression of nuclear factor kB (NF-kB) and intercellular adhesion molecule-1 (ICAM-1) in the lung tissue damaged by gas explosion and their relationship. Methods The rat model of gas explosion was established. The expression of NF-κB mRNA in lung tissue was detected by digoxigenin-labeled NF-κB cDNA probe. The expression of NF-κB and ICAM-1 in lung tissue was detected by immunohistochemical SP method. Results After the gas explosion, the expression of NF-kB mRNA, protein and ICAM-1 in the lung tissue of rats in each experimental group increased significantly compared with the control group, and the most obvious increase occurred at 2 h after injury. Lung histopathological examination showed that after gas explosion injury, some tracheal mucosal epithelial shedding, wall congestion, edema, neutrophil infiltration; alveolar hemorrhage, edema, a large number of inflammatory cell infiltration. Electron microscopy showed type Ⅱ alveolar epithelial cells degeneration, changes significantly. Conclusions Gas explosion caused NF-kB activation and promoted the expression of ICAM-1, which is closely related to acute lung injury.