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目的:研究探讨独活干预血管性痴呆模型大鼠自由基和免疫炎性损伤的临床作用。方法:选取健康雄性SD大鼠80只作为研究对象,并将其随机分为五组,分别将其设为空白对照组、假手术组、模型对照组、中药组和西药组,对后三组大鼠建立血管性痴呆模型,分别在造模前、造模后1周、给药后等不同时期对大鼠进行水迷宫实验,比较其不同阶段的逃避潜伏期,并在最后一次水迷宫实验结束之后,对大鼠实施解剖,获得腹主动脉血液和海马组织,分别处理后,对五组大鼠的血清中、海马CA1区内TNF-α表达水平、血清中SOD活性或MDA表达值等进行检测和比较。结果:造模前后不同时期各组大鼠的逃避潜伏期比较可见,中药组和西药组给药后逃避潜伏期显著缩短,组内不同时期的比较、与其他三组的比较均有统计学差异(P<0.05)。大鼠血清中和海马CA1区TNF-α的表达情况的比较,均有模型对照组TNF-α的表达水平最高,其次是中药组和西药组,而空白对照组和假手术组最低的规律,比较均有统计学差异(P<0.05)。模型对照组的SOD值最低,中药组和西药组略高,空白对照组和假手术组最高,MDA表达值的比较规律则正好相反。结论:独活能有效改善血管痴呆模型大鼠自由基和免疫炎性损伤情况,提高脑组织氧自由基的代谢,促进大鼠的学习与记忆能力的恢复,效果良好。
Objective: To investigate the clinical effect of Jurchen intervention on free radical and immunoinflammatory injury in vascular dementia model rats. Methods: Eighty healthy male Sprague-Dawley rats were selected as research objects and randomly divided into five groups, which were set as blank control group, sham operation group, model control group, traditional Chinese medicine group and western medicine group, Rats were established vascular dementia model, respectively, before modeling, 1 week after modeling, after administration of water maze test at different stages, compared with different stages of the escape latency and water maze test at the end of the last After that, the rats were dissected and the abdominal aorta blood and hippocampus were obtained. After treatment, the expression of TNF-α, the activity of SOD or MDA in the hippocampal CA1 area of the five groups of rats Test and compare. Results: Compared with the other three groups, the escape latency of rats in each group was significantly reduced at different periods before and after modeling. The escape latency of TCM and western medicine groups was significantly shortened after administration <0.05). The expression of TNF-α in the rat serum and hippocampal CA1 region was the highest in the model control group, followed by the traditional Chinese medicine group and western medicine group, while the lowest in the blank control group and sham operation group, The difference was statistically significant (P <0.05). The model control group had the lowest SOD value, the Chinese medicine group and the western medicine group were slightly higher, the blank control group and sham operation group were the highest, and the comparison rule of MDA expression value was the opposite. CONCLUSION: Joguang can effectively improve the free radical and immunoinflammatory injury of vascular dementia model rats, improve the metabolism of oxygen free radicals in brain tissues and promote the recovery of learning and memory ability in rats.