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目的:探讨盐酸四环素(Tc)对去卵巢大鼠骨代谢和子宫的影响。方法:假手术组、去卵巢组、Tc1组(1.2 mg·kg~(-1)·d~(-1)),Tc2组(4.8mg·kg~(-1)·d~(-1)),雌酮组(1.48mg·kg~(-1)·d~(-1)),灌胃90天。胫骨上段不脱钙骨制片测量。结果:(1)去卵巢溶剂组骨小梁面积减少,出现骨高转化的改变。(2)与去卵巢组比,雌酮能部分预防骨丢失,降低骨高转化,有明显增加子宫重量的副作用。(3)Tc两组均能部分预防去卵巢所致的骨丢失,未出现骨高转化的改变,未见增加子宫重量的副作用。与去卵巢组比,在骨形成的参数中,Tc1组降低矿化沉积率。而Tc2组降低荧光周长百分率、骨形成率,即其抑制骨形成的作用比Tc1组大。结论:低剂量盐酸四环素能预防去卵巢所致的骨丢失,高剂量未出现更强的增加骨量作用,两剂量四环素均未出现明显肝、肾和子宫的副作用。
Objective: To investigate the effect of tetracycline hydrochloride (Tc) on bone metabolism and uterus in ovariectomized rats. Methods: Tc2 group (4.8 mg · kg -1 · d -1) was administered with Tc 1 (1.2 mg · kg -1 · d -1), sham operation group, ovariectomized group, ) And estrone group (1.48 mg · kg -1 · d -1) for 90 days. Upper tibia non-decalcified bone slices measured. Results: (1) The area of trabecular bone in ovariectomized group decreased, and the change of bone hyperplasia appeared. (2) Compared with the ovariectomized group, estrone could partly prevent the bone loss, reduce the high bone turnover, and significantly increase the side effects of uterine weight. (3) Both groups of Tc can partially prevent the loss of ovaries caused by bone loss, there is no change of bone hyperplasia, no increase of uterine weight side effects. Compared with the ovariectomized group, the Tc1 group reduced the mineralized deposition rate in the parameters of bone formation. The Tc2 group decreased the percentage of fluorescence perimeter, bone formation rate, that is, its role in inhibiting bone formation larger than the Tc1 group. CONCLUSION: Tetracycline hydrochloride can prevent bone loss caused by ovariectomy without increasing the bone mass in high dose. No obvious side effects of liver, kidney and uterus are found in the two doses of tetracycline.