【摘 要】
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Dear Editor,The recent development of sequence-specific nuclease systems,i.e.,TALENs and CRISPR/Cas9,has made genomic targeting easier in many organisms includi
【机 构】
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State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 51
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Dear Editor,The recent development of sequence-specific nuclease systems,i.e.,TALENs and CRISPR/Cas9,has made genomic targeting easier in many organisms including plants (Li et al.,2012;Cong et al.,2013;Joung and Sander,2013;Li,et al.,2013;Shan et al.,2013;Liang et al.,2014;Zhang et al.,2014).Mutations induced by CRISPR/Cas9 usually occur around the cleavage sites at three bases upstream of the protospacer-adjacent motif (PAM),producing insertion and deletion of nucleotides.For diploid organisms,such targeted mutations may happen in one or both homologous chromosomes.
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