论文部分内容阅读
目的:观察复方甘草甜素对肺纤维化大鼠血清IFN-γ、IL-4表达水平的影响,探讨复方甘草甜素对肺纤维化的治疗作用及可能的作用机制。方法:72只SD健康大鼠,随机分为4组(每组各18只):空白对照组、模型对照组、地塞米松阳性对照组、甘草甜素治疗组。模型对照组、地塞米松阳性对照组和甘草甜素治疗组气管内注射博莱霉素诱导肺纤维化模型,空白对照组在相同条件下给予生理盐水。甘草甜素治疗组和地塞米松阳性对照组每天分别给予复方甘草甜素2mL/100g腹腔内注射,地塞米松注射液0.03mg/100g腹腔内注射,其余两组每天生理盐水2mL/100g腹腔内注射。各组分别于造模后第7、14、28天各处死6只大鼠,取大鼠血清和肺组织。肺组织病理切片HE染色和MASSON染色观察病理变化和肺纤维化分级情况,ELISA法测定血清中INF-γ、IL-4含量的变化。结果:与模型对照组比较,甘草甜素治疗组各个时期的血清IFN-γ表达明显增高(P<0.05);地塞米松阳性对照组大鼠血清IL-4在各个时期的表达降低明显(P<0.05),尤其在第7天下降明显,比甘草甜素治疗组更低(P<0.05)。结论:复方甘草甜素对肺纤维化的形成有较好的治疗作用,其机制可能是早期通过促进血清中IFN-γ的分泌,抑制IL-4的分泌,从而调节了Th1/Th2细胞因子失衡,减轻肺泡炎来实现的,后期通过上调INF-γ水平对肺纤维化的抑制作用。
Objective: To observe the effects of compound glycyrrhizin on the serum levels of IFN-γ and IL-4 in rats with pulmonary fibrosis, and to explore the therapeutic effect of compound glycyrrhizin on pulmonary fibrosis and its possible mechanism. Methods: Seventy-two SD healthy rats were randomly divided into 4 groups (18 in each group): blank control group, model control group, dexamethasone positive control group and glycyrrhizin treatment group. The model control group, the dexamethasone positive control group and the glycyrrhizin treatment group were injected intratracheally with bleomycin-induced pulmonary fibrosis model, and the blank control group were given normal saline under the same conditions. Glycyrrhizin treatment group and dexamethasone positive control group were given compound glycyrrhizin 2mL / 100g intraperitoneal injection, dexamethasone injection 0.03mg / 100g intraperitoneal injection, the other two groups daily saline 2mL / 100g intraperitoneal injection. Six rats were sacrificed on the 7th, 14th and 28th day after the model was established, respectively. Serum and lung tissue were obtained from the rats. Pathological changes of lung tissue and classification of pulmonary fibrosis were observed by HE staining and MASSON staining. The changes of INF-γ and IL-4 in serum were detected by ELISA. Results: Compared with the model control group, the expression of IFN-γ in the glycyrrhizin treated groups was significantly increased at each time point (P <0.05); the expression of IL-4 in the dexamethasone positive control group decreased significantly at all stages (P <0.05), especially in the seventh day decreased significantly (P <0.05) than glycyrrhizin treatment group. CONCLUSION: Compound glycyrrhizin has a good therapeutic effect on the formation of pulmonary fibrosis. The mechanism may be that it may regulate the imbalance of Th1 / Th2 cytokines by promoting the secretion of IFN-γ in serum and inhibiting the secretion of IL-4 , Reduce alveolitis to achieve, late by inhibiting the level of INF-γ on pulmonary fibrosis.