论文部分内容阅读
目的:探讨大鼠骨髓间充质干细胞(mesenchymalstemcells,MSCs)在诱导条件下的成骨特性。方法:使用密度梯度法分离成年大鼠骨髓MSCs,以地塞米松、β-甘油磷酸钠、维生素C为成骨诱导剂。结果:形态学表明,MSCs贴壁细胞呈集落生长,有成纤维细胞外观。地塞米松低剂量组诱导7d后碱性磷酸酶(alkalinephosphatase,ALP)表达明显升高,阳性细胞数为(15.1±2.6)个,而对照组ALP表达较弱,阳性细胞数为(12.0±3.5)个,两组比较P<0.01。地塞米松成骨诱导剂低剂量组促进MSC成骨,在诱导7d时即出现钙化结节,而对照组未出现;地塞米松低剂量组诱导21d钙化结节为(9.0±1.7)个,而对照组为(2.0±1.8)个,两组比较P<0.01。结论:低剂量地塞米松成骨诱导剂能诱导MSC向成骨细胞分化,可以为体内骨组织工程提供种子细胞。
Objective: To investigate the osteogenic properties of rat mesenchymal stem cells (MSCs) under induction. METHODS: Adult rat bone marrow MSCs were isolated using density gradient method. Dexamethasone, β-glycerophosphate and vitamin C were used as osteoinductive agents. Results: Morphology showed that adherent cells of MSCs showed colony growth with the appearance of fibroblasts. The expression of alkaline phosphatase (ALP) was significantly increased in the dexamethasone low-dose group for 7 days after induction, the number of positive cells was (15.1 ± 2.6), while the control group showed weak ALP expression (12.0 ± 3.5 ), P <0.01 for both groups. Dexamethasone osteoinductive agent low-dose group can promote MSC osteogenesis, calcified nodules appeared on the 7th day, while the control group did not appear; dexamethasone low dose group induced 21d calcified nodules (9.0 ± 1.7) Compared with (2.0 ± 1.8) in the control group, P <0.01 in both groups. CONCLUSION: Low dose dexamethasone osteoinductive agent induces MSC differentiation into osteoblasts and can provide seed cells for in vivo bone tissue engineering.