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本研究用２０只纯种日本大耳白幼兔，实验组连续二次、间隔２４ｈ经静脉注入纯制大肠杆菌内毒素（１００μｇ／ｋｇ），建立革兰氏阴性菌败血症休克模型，二周末处死，以９９ｍＴｃ－ＭＤＰ骨显像法及病理学检查观察模型动物骨骼系统改变情况，评价ＤＩＣ过程对骨骼系统的影响。结果发现，在第二次静脉注射内毒素一周后，实验组动物长骨骨端血流量计数对比值显著下降，二周末病理检查，证明出现了多发性骨坏死，并集中地发生在长管状骨骨端，分别为股骨髁、肱骨头、胫骨上端、股骨头及尺骨近端。病理表现为骺及干骺端侧髓腔内骨髓细胞坏死，以及骺板干骺端侧的软骨细胞柱、新生及成熟骨小梁的坏死崩解。部分动物有整块骺板的变性及坏死。实验发现，骨坏死的发生和分布，与骨微循环结构的破坏密切相关。这表明，内毒素及其引发的ＤＩＣ反应可以破坏骺区的骨微循环系统，引起幼兔全身多发的以骺板为中心的骨坏死。本研究提示，内毒素及其引发的ＤＩＣ过程可能是严重革兰氏阴性菌败血症后，儿童后遗骨发育障碍的重要原因
In this study, 20 purebred Japanese white rabbits were used. The experimental group was injected twice with pure E. coli endotoxin (100μg / kg) intravenously 24 hours apart to establish Gram-negative septic shock model. The changes of skeletal system in model animals were observed by 99mTc-MDP bone imaging and pathological examination, and the effect of DIC on skeletal system was evaluated. The results showed that after a second intravenous injection of endotoxin for one week, the experimental group, long bone and bone side of the blood flow count decreased significantly, two weeks of pathological examination showed that multiple osteonecrosis occurred, and concentrated in the long tubular bone End, respectively femoral condyle, humeral head, tibia, femoral head and proximal ulna. Pathological findings of epiphyseal and metaphyseal medullary bone marrow necrosis, and epiphyseal metaphyseal side of the cartilage column, neonatal and mature trabecular bone necrosis. Some animals have the entire epiphyseal plate degeneration and necrosis. The experiment found that the occurrence and distribution of osteonecrosis and the destruction of bone microcirculation structure are closely related. This indicates that endotoxin and its induced DIC reaction can destroy the bone microcirculation system in the epiphyseal area and cause the multiple epiphyseal plate-centered osteonecrosis of the young rabbits. This study suggests that endotoxin and its induced DIC may be an important cause of post-bony development disorder in children after severe Gram-negative septicemia