论文部分内容阅读
建立了高效毛细管电泳法测定乳氟禾草灵的含量。使用有效长度为42cm、内径为50μm的毛细管,在背景电解质为50mmol/L硼砂-150mmol/L磷酸二氢钠(pH值9.5)、检测波长为281nm、分离电压为15kV的优化条件下,分析时间小于6min。乳氟禾草灵质量浓度在10～240mg/L内线性关系良好,相关系数为0.9997;方法的检测限(S/N为3)和定量限(S/N为10)分别为0.8、2.0mg/L;加标回收率为98.2%～99.0%,相对标准偏差为0.59%～2.6%。
A high performance capillary electrophoresis was established to determine the content of lactofen. Using capillary with an effective length of 42cm and an inner diameter of 50μm, under the optimized conditions that the background electrolyte is 50mmol / L borax-150mmol / L sodium dihydrogen phosphate (pH 9.5), the detection wavelength is 281nm and the separation voltage is 15kV, Less than 6min. The linear range of the concentration of lactoferricin was 10 ~ 240mg / L with a correlation coefficient of 0.9997. The limits of detection (S / N 3) and limit of quantification (S / N 10) of the method were 0.8 and 2.0 mg / L; the spiked recoveries ranged from 98.2% to 99.0% with relative standard deviations of 0.59% -2.6%.