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单克隆抗体(下称单抗)由于仅有唯一特异抗原结合位点,其标记较多克隆抗体困难,尤其应用IgM单抗则更为困难。作者用戊二醛二步法,过碘酸钠氧化法。和戊二醛-过碘酸钠氧化法标记IgM单抗均未成功。而用改良过碘酸钠氧化法得到满意效果。
Monoclonal antibodies (MAbs) are more difficult to label than monoclonal antibodies because there is only a unique antigen-binding site, and it is even more difficult to use IgM mAbs in particular. Author with glutaraldehyde two-step method, sodium periodate oxidation. And glutaraldehyde - sodium periodate oxidation of IgM monoclonal antibody were not successful. Satisfactory results are obtained with the improved sodium periodate oxidation method.