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为验证微生物检测实验室压力蒸汽灭菌的技能和对微生物检验结果准确性的监控,2007年由浙江省质量技术监督局组织,杭州市疾病预防控制中心承办,杭州致远医学检验所有限公司制备提供考核样品,在全省取得计量认证资格的实验室范围内开展了压力蒸汽灭菌技能考核。由组织方统一提供压力蒸汽灭菌考核样和压力蒸汽灭菌效果检测专用培养基,统一操作方法,全省各行业共81家实验室参加考核。结果合格有41家,占总数的50.62%;结果不合格的40家,占49.38%。考核结果表明,目前微生物实验室的热力灭菌的基本理论知识较差,在操作过程中没有编制灭菌工艺指导书,没有严格遵守操作规程,实际工作中未应用生物指示剂(压力蒸汽灭菌效果检测标样等)定期检测灭菌效果和复验灭菌工艺的各种技术参数,直接影响微生物检测结果。
In order to verify the technology of pressure steam sterilization in microbiological testing laboratory and the monitoring of the accuracy of microbiological test results, it was organized by Zhejiang Quality and Technical Supervision Bureau, undertaken by Hangzhou Center for Disease Control and Prevention in 2007 and prepared by Hangzhou Zhiyuan Medical Laboratory Co., Ltd. Assessment samples, in the province to obtain accreditation metrology qualification laboratory carried out pressure steam sterilization skills assessment. The organizers provide uniform pressure steam sterilization test sample and pressure steam sterilization effect detection medium, a unified method of operation, the province’s various industries a total of 81 laboratories to participate in the assessment. The results of qualified 41, accounting for 50.62% of the total; 40 failed results, accounting for 49.38%. The examination results show that the basic theoretical knowledge of thermodynamic sterilization in microbial laboratories is poor. There is no guideline for preparing sterilization procedures during the operation, and the operation procedures are not strictly followed. In practical work, no biological indicator (pressure steam sterilization Effect detection standards, etc.) Regularly check the sterilization effect and retest sterilization of a variety of technical parameters, a direct impact on microbial test results.