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目的:探讨眼针改善脑缺血再灌注损伤的机制.方法:健康SD大鼠32只按随机数字表分正常组、假手术组、模型组和眼针组4组,每组8只.模型组及眼针组应用线栓法复制脑缺血再灌注损伤大鼠模型.眼针组于脑缺血再灌注即刻、12h及23.5 h,取肝区、上焦区、下焦区、肾区进行眼针治疗20 min.正常组未进行处理；假手术组插入栓塞线深度0.5～1.0 cm,其余同模型组.于再灌注即刻及24 h进行神经功能缺损评分,采用Western blot、实时荧光定量聚合酶链反应(RT-PCR)方法测定右侧海马组织脑源性神经营养因子( BDNF)蛋白及mRNA表达.结果:再灌注即刻眼针组与模型组大鼠神经功能缺损评分无差别,再灌注24 h眼针组评分为1.50±0.53,与模型组3.13±0.64比较,明显下降(P＜0.01)；Western blot检测海马组织BDNF蛋白表达为正常组0.71±0.02、假手术组0.70±0.04、眼针组0.69±0.04,与模型组0.37±0.03比较均有统计学差异(P＜0.01)；各组大鼠BDNF mRNA表达趋势同蛋白表达.结论:眼针疗法能通过增加内源性BDNF的表达以促进大鼠脑缺血再灌注损伤过程中神经干细胞的修复,实现对脑缺血组织保护的目的.“,”Objective To observe the effects of eye acupuncture on expression of brainderived neurotrophic factor (BDNF) in the hippocampus of rats after ischemiareperfusion injury in order to study the mechanisms of eye acupuncture in improving ischemia-reperfusion injury.Methods SD rats were randomly divided into a normal group,a sham-operation group,a model group and an eye acupuncture group,with 8 rats in each.The cerebral ischemiareperfusion injury model was established by thread occlusion method.Acupuncture at “liver”,“upper-jiao”,“lower-jiao”,and “kidney” was performed for 20 min in the eye acupuncture group immediateness,12 h and 23.5 h after the reperfusion.After 24 h of the reperfusion,the neurophysical behaviors were evaluated by Zea Longa neurophysical impairment score; the expression of ischemic hippocampus BDNF mRNA was measured by RTPCR; the expression of ischemic hippocampus BDNF protein was detected by Western blot technique.Results After reperfusion 24 h,compared with the model group,the neurologic impairment score of the eye acupuncture group decreased significantly (P＜0.01); the expressions of BDNF mRNA and protein in rat hippocampi after the eye acupuncture therapy were both obviously increased (P＜0.01).Conclusion The eye acupuncture therapy is beneficial for neurofunctional rehabilitation by promoting the repair of the nerve cell which is induced by increasing hippocampus BDNF expression.