建立了溶菌酶复性的表观动力学模型 ,研究了分子伴侣GroEL促进变性溶菌酶复性的动力学行为 ,包括酶浓度、三磷酸腺苷 (ATP)浓度、GroEL与酶的摩尔比对复性率和复性速率常数的影响 .酶浓度对复性速率常数的影响比对复性率的影响显著 ;酶的复性率和复性速率常数随ATP浓度的增大而提高 ;在蛋白质浓度一定的情况下 ,存在适宜的GroEL和ATP浓度 ,使复性率和复性速率常数最大 The apparent kinetic model of lysozyme renaturation was established and the kinetic behavior of renaturation of denatured lysozyme by molecular chaperone GroEL was studied. The kinetic behavior of denatured lysozyme renaturation was studied, including enzyme concentration, adenosine triphosphate (ATP) concentration, molar ratio of GroEL to enzyme, The effect of enzyme concentration on the refolding rate constant was more significant than that on the refolding rate. The refolding rate and refolding rate constant of the enzyme increased with the increase of ATP concentration. When the protein concentration was constant , There is a suitable concentration of GroEL and ATP to maximize the refolding rate and refolding rate constant