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目的探讨妊娠期接触苯对其子鼠免疫功能的影响。方法 40只孕鼠被随机等分为空气对照组和5.0、10.01、5.0 mg/m3三个不同浓度的苯染毒组,各组从孕7 d开始,连续染毒至分娩,每天染毒2 h。分别在分娩后1 d及7d,每组取5只母鼠和5只子鼠取血检测T淋巴细胞亚群和血清免疫球蛋白的含量。结果在子鼠出生后1 d和7 d,等浓度组的母鼠和子鼠的血T淋巴细胞亚群、血免疫球蛋白的含量差异均无统计学意义(P>0.05);低浓度苯染毒组与对照组比较,CD3+、CD4+、CD4+/CD8+和血各种免疫球蛋白的含量差异均无统计学意义(P>0.05);但在中、高浓度组CD3+、CD4+和CD4+/CD8+和血各种免疫球蛋白的含量均较对照组低(P<0.05),并呈明显的剂量-效应关系(P<0.01)。但无论是母鼠还是子鼠,在两个时间点间的T淋巴细胞亚群和血各种免疫球蛋白的含量变化差异无统计学意义(P>0.05)。结论妊娠期鼠吸入苯可抑制母鼠及其子鼠细胞免疫和体液免疫功能。
Objective To investigate the effect of exposure to benzene on the immune function of its offspring in pregnancy. Methods Forty pregnant rats were randomly divided into three groups: air control group and benzene exposure group at 5.0, 10.01 and 5.0 mg / m3 respectively. Each group started from 7th day of pregnancy with continuous exposure to childbirth, h At 1 d and 7 d after delivery, 5 maternal and 5 offspring from each group were taken to detect T lymphocyte subsets and serum immunoglobulins. Results There was no significant difference in blood T lymphocyte subsets and blood immunoglobulins between the same concentration of dams and offspring on day 1 and day 7 after birth (P> 0.05). Low concentration of benzene dye The levels of CD3 +, CD4 +, CD4 + / CD8 + and immunoglobulin in blood were not significantly different between the poisoned and control groups (P> 0.05). However, the levels of CD3 +, CD4 + and CD4 + / CD8 + The contents of various immunoglobulin in blood were lower than those in control group (P <0.05), and showed significant dose-effect relationship (P <0.01). However, there was no significant difference in the contents of T lymphocyte subsets and various immunoglobulins between the two groups at either time point (P> 0.05). Conclusion Inhalation of benzene in pregnant rats inhibits the cellular immunity and humoral immunity of pregnant rats and their offspring.